食用糖浆及其原料转基因成分的荧光PCR检测  

Detection of Genetically Modified Ingredient in Edible Syrup and Corn Starch by Real-Time Fluorescence PCR Method

作　　者：闫超杰 张旭东 付海滨[2,3] 李俊环 张敏[4] Yah Chaojie;Zhang Xudong;Fu Haibin;Li Junhuan;Zhang Min(Jinzhou Entry Exit Inspection and Quarantine Bureau, Jinzhou 121013, China;Shenyang Entry Exit Inspection and Quarantine Bureau, Shenyang 110016, China;Shenyang Academy of Inspection and Quarantine, Shenyang 110016, China;Shenyang Agricultural University, Shenyang 110866, China)

机构地区：[1]锦州出入境检验检疫局,辽宁锦州121013 [2]沈阳出入境检验检疫局,辽宁沈阳110016 [3]沈阳检验检疫科学研究院,辽宁沈阳110016 [4]沈阳农业大学,辽宁沈阳110016

出　　处：《沈阳大学学报（自然科学版）》2018年第2期100-104,共5页Journal of Shenyang University：Natural Science

基　　金：辽宁省大型科学仪器共享平台管理项目(2017-2018);沈阳市2016年科技计划资助项目(F16-104-4-00)

摘　　要：采用加大取样量(取10管共50mL样品)、PBS缓冲液预处理、深加工食品DNA试剂盒核酸提取、富集DNA溶液总体积、加大反应体系DNA模板量的方法,成功提取到了糖浆制品的内源基因,建立了食用糖浆中转基因成分实时荧光PCR定性检测方法.同时,对糖浆原料(玉米淀粉)进行转基因成分检测,结果83.3%样品均检出外源基因成分.The endogenous gene of edible syrup was extracted successfully in this experiment by increasing sample amount （10 tubes of 50 mL samples）, PBS buffer pretreatment, using DNA extraction kit for highly processing of food, enriching the total volume of DNA solution, and increasing the amount of DNA template in the reaction system. Real time fluorescence quantitative PCR method for the detection of transgenes in edible syrup was established. Genetically modified ingredient of corn starch samples （processed materials of edible syrup） were detected in the process of experiment. The results show that, foreign genes are detected in 83.3% samples.

关 键 词：食用糖浆 玉米淀粉 转基因食品 DNA提取 实时荧光PCR 

分 类 号：Q78[生物学—分子生物学]