香蕉茎叶生物活性鱼用饲料的发酵工艺优化  

Fermentation Process Optimization of Banana Stems and Leaves as Bioactive Fish Feed

作　　者：张玲 蔡艺英 梁妍 李春海 ZHANG Ling;CAI Yiying;LIANG Yan;LI Chunhai(Technology Research Center for Lingnan Characteristic Fruits ＆ Vegetables Processing and Application Engineering of Guangdong Province, Food Science Innovation Team of Guangdong Higher Education Institutes, Development Center of Technology for Fruit ＆ Vegetable Storage and Processing Engineering, Guangdong University of Petrochemical Technology, Maoming 525000 China;College of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China)

机构地区：[1]广东省岭南特色果蔬加工及应用工程技术研究中心广东普通高校食品科学创新团队广东高校果蔬加工与贮藏工程技术开发中心,广东茂名525000 [2]广东石油化工学院环境与生物工程学院,广东茂名525000

出　　处：《广东石油化工学院学报》2018年第1期12-17,共6页Journal of Guangdong University of Petrochemical Technology

基　　金：广东省科技计划项目(香蕉茎叶/光合细菌生物活性鱼用饲料的研制与应用);广东省岭南特色果蔬加工关键技术及应用工程技术研究中心项目(粤科函产学研字[2015]1487号);广东普通高校食品科学创新团队项目(2016KCXTD020);广东高校果蔬加工与贮藏工程技术开发中心项目(2012gczx B001)

摘　　要：以新鲜香蕉茎叶为原料,植物乳杆菌及光合细菌为发酵剂,研制了一种具有生物活性的鱼用青贮饲料,并对发酵工艺条件进行了优化。以饲料蛋白质含量及感官综合评分为依据,采用单因素试验考查了蔗糖添加量、混合菌种比例、接种质量分数、发酵时间和温度5个单因素对饲料品质的影响;采用正交试验优化最佳发酵工艺条件。结果表明,最佳发酵条件为蔗糖添加质量分数2.5%、光合菌种与植物乳杆菌质量比为1∶3、接种质量分数为3%,在33℃下密封发酵7 d。此条件下制得的饲料蛋白质质量分数为9.66%,具有独特的芳香气味,茎叶结构保持良好,无霉腐,烘干后为褐色。In this experiment, fresh banana stems and leaves are used as raw materials, the lactobacillus plantamm and photosyn-thetic bacteria as fermentation agents, and a kind of bioactive fish feed is developed, with fermentation process conditions optimized. Based on the feed protein content and sensory comprehensive score, the single factor experiments are used to test the effects of su-crose content, ratio of mixed strains and inoculum amount, fermentation time and temperature on the quality of feed. The optimum fermentation conditions are optimized by orthogonal experiments. The results show that the optimal fermentation conditions are as fol-lows ：the sucrose content 2. 5% ,the mixed strains inoculation 3% , and the mass ratio of mixed strains 1＊ 3 （photosynthetic bacte-ria： Lactobacillus plantamm） , sealed fermentation under 33 °C for 7 d. Under this condition, the protein in this fodder of banana stems and leaves reaches 9. 66% , and the fodder in which the stem and leaf structure remain well has a unique aroma, without any mold or rot, and is in brown after being dried.

关 键 词：香蕉茎叶 饲料 植物乳杆菌 光合细菌 发酵 蛋白质 

分 类 号：S963[农业科学—水产养殖] S59[农业科学—水产科学]