花生芽茎白藜芦醇的酶解辅助提取工艺  

Optimization of Enzymolysis Assisted Extraction Process Conditions of Resveratrol from Peanut Buds

作　　者：陶志杰[1] 王改玲[1] 项蕊 钱时权[1] 柯春林[1] TAO Zhi-jie;WANG Gai-ling;Xiang Rui;QIAN Shi-quan;KE Chun-lin(School of Biological and Food Engineering, Bengbu University,Bengbu,233030,Anhu)

机构地区：[1]蚌埠学院生物与食品工程学院,安徽蚌埠233030

出　　处：《蚌埠学院学报》2018年第2期14-18,共5页Journal of Bengbu University

基　　金：安徽省大学生创新创业训练计划项目(AH201611305065);安徽省高校自然科学研究项目(113052015KJ07);生物化学与分子生物学院级重点学科项目(院字[2014]155号)

摘　　要：研究酶解辅助乙醇提取花生芽茎白藜芦醇的最佳工艺条件,以纤维素酶为辅助酶,通过单因素试验确定酶解时间、酶用量、酶解温度对白藜芦醇提取量的影响,利用Box-Benhnken试验设计和响应面法分析。在酶解pH为5.0,料液比为1∶10的条件下,确定最佳酶解条件为酶解时间65 min、酶用量3.12 mg/g、酶解温度55℃。在此条件下,白藜芦醇平均提取量为125.785μg/g,较为接近预测值。从而得出,响应面法优化后得出的最佳酶解条件有实践指导价值。The objective of this study was to optimize enzymatic-assisted ethanol extraction of resveratrol from peanut buds. Cellulase were used in this paper on the basis of Single-factor test,the Response Surface Methodology was used to investigate the effects of processing parameters,including enzymatic time,enzyme dosage and enzymatic temperature. Under the conditions of enzymatic hydrolysis pH 5. 0 and the solid-liquid ratio 1∶ 10,the optimum conditions were enzymatic hydrolysis time 65 min,enzyme dosage 3. 12 mg/g,hydrolysis temperature 55 ℃. Under these conditions,the average content of resveratrol was125. 785 μg/g. The result was close to the theoretical value and well suggests production prediction.

关 键 词：花生芽 白藜芦醇 纤维素酶 响应面法 

分 类 号：Q813.1[生物学—生物工程]