CXCL5通过上调Cyclin D1促进卵巢癌细胞增殖  

CXCL5 promote ovarian cancer cells proliferation by upregulating cyclin D1 expression

作　　者：建方方[1] 刘华[1] 冯炜炜 王岳萍[1] 沈立翡[1] Jian Fangfang;Liu Hua;Feng Weiwei(Department of Obstetrics and Gynecology, Ruifin Hospital, Shanghai Jiaotong University School of Medicine ,Shanghai 20002)

机构地区：[1]上海交通大学医学院附属瑞金医院妇产科,上海200025

出　　处：《现代妇产科进展》2018年第4期255-258,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:研究CXCL5对卵巢癌细胞增殖的影响及其分子机制。方法:Western blot法检测卵巢癌细胞株中CXCL5表达;构建CXCL5敲低和过表达稳转卵巢癌细胞株,Western blot法验证效率。平板克隆试验、CCK-8、流式细胞术检测CXCL5对卵巢癌细胞增殖能力的影响。Western blot法检测细胞增殖相关蛋白及Akt和Erk等上游分子表达。结果:Western blot法结果显示,CXCL5在卵巢癌细胞株中表达。敲低CXCL5表达后,卵巢癌细胞增殖能力较对照组明显下降(P<0.05);过表达CXCL5卵巢癌细胞的增殖能力较对照组明显提高(P<0.05)。敲低CXCL5表达后Cyclin D1表达下调,过表达CXCL5后Cyclin D1表达上调,差异均有统计学意义(P<0.05),而Cyclin C和Cyclin E表达无显著变化。敲低CXCL5表达后,p-Erk表达显著下调,过表达CXCL5后p-Erk表达显著上调,差异均有统计学意义(P<0.05);p-Akt表达无显著变化。结论:CXCL5促进卵巢癌细胞增殖,其可能机制是通过激活Erk通路引起周期蛋白Cyclin D1表达上调。Objective： To study the influence of CXCL5 on the proliferation of ovarian cancer cells and the mechanism involved.Methods： Expression of CXCL5 in ovarian cancer cell lines was examined by Western blot.The lentiviral vectors LV5-EF1 a-GFP/Puro-CXCL5 and LV3-p GLV-h1-GFP/Puro-sh CXCL5 were transfected into ovarian cancer cells. The efficiency of transfection was verified by Western blot.The proliferation of ovarian cancer cells were detected by colony formation assay,CCK-8 assay and flow cytometry.Expression of proliferation-related proteins was detected to reveal the mechanism of CXCL5 in promoting ovarian cancer proliferation.Results： CXCL5 was expressed in ovarian cancer cell lines.In CXCL5-silenced cells,the proliferation ability was significantly decreased compared with the control groups,while overexpression of CXCL5 increased the proliferation of ovarian cancer cells.CXCL5 increased the proliferation of ovarian cancer cells by up-regulating Cyclin D1.Conclusion： CXCL5 increases the proliferation of ovarian cancer cells by up-regulating Cyclin D1.

关 键 词：CXCL5 卵巢癌 细胞增殖 CYCLIN D1 

分 类 号：R737.33[医药卫生—肿瘤]