氧载体对绿色产色链霉菌发酵生产阿维拉霉素的影响  

Effects of oxygen vectors on microbial production of avilamycin

作　　者：章梅 宋元达[1,2] 刘青 王友玲 吴翰 ZHANG Mei,SONG Yuan-da,LIU Qing,WANG You-ling,WU Han(1. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255000, China ; 2. Key Laboratory of Technologies for Functional Agricultural Products in Universities of Shandong , Zibo , Shandong 255000, Chin)

机构地区：[1]山东理工大学农业工程与食品科学学院,山东淄博255000 [2]山东省高校农产品功能化技术重点实验室,山东淄博255000

出　　处：《食品与机械》2018年第2期42-45,128,共5页Food and Machinery

基　　金：国家自然科学基金(编号:31670064);江苏凌云药业"链霉菌基因工程改造横向合作项目"(编号:2016-科技-90)

摘　　要：为了提高阿维拉霉素发酵液中氧传递效率,改善发酵液中溶氧水平,增加阿维拉霉素的产量,研究各种氧载体对发酵过程中阿维拉霉素的合成和绿色产色链霉菌的影响。试验结果显示,吐温80作为氧载体的效果最好,能有效地提高生物量和单位菌体的阿维拉霉素的产率。在发酵开始的8h,添加3g/L吐温80,生物量达到了11.82g/L,阿维拉霉素效价达到了340mg/L,与对照组比较,分别提高了20.63%和21.42%,研究表明氧载体吐温80能通过提高生物量和得率系数来提高阿维拉霉素的产量。In order to improve oxygen delivery efficiency and dissolved oxygen levels for the production of avilamycin in the fermentation broth,the effects were studied,including the various oxygen carriers on the synthesis of avilamycin and the growth of Streptomyces viridochromogenes.The results showed that Tween-80 was the most effective vector to promote growth and the ability of Streptomyces viridochromogene to produce avilamycin.With the addition of 3 g/L tween at 8 hfermentation,the biomass reached 11.82 g/L and the avilamycin titer increased to 340 mg/L,which was 20.63% and21.42% higher than the control group.Result show that the oxygen carrier of Tween-80 can increase the production of avilamycin by increasing the biomass and yield coefficient.

关 键 词：绿色产色链霉菌 阿维拉霉素 氧载体 发酵 

分 类 号：TQ927[轻工技术与工程—发酵工程]