异养小球藻发酵液上清液回用的试验研究  

Reuse of liquid supernatant in the fermentation broth of allotrophic Chlorella vulgaris

作　　者：吴娟[1,2] 姚伦广[3] 毕生雷[2] 鲁龙 张鹏飞[2] 陈斯楠[1,2] WU Juan1,2, YAO Lunguang1, BI Shenglei2 ,LU Long2 , ZHANG Pengfei2, CHEN Sinan1,2(1. Key Laboratory of Ecological Security for Water Source Region of Mid - line of South - to - North Diversion Project of Henan Province, Collaborative Innovation Center of Water Security for Water Source Region of Mid - line of South - to - North Diversion Project of Henan Province, Nanyang Normal University, Nanyang 473000, Henan, China; 2. State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Group Co. , Ltd. , Nanyang 473000, Henan, Chin)

机构地区：[1]南阳师范学院南水北调中线水源区水安全河南省协同创新中心,河南省南水北调中线水源区生态安全重点实验室,河南南阳473000 [2]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [3]南阳师范学院南水北调中线水源区水安全河南省协同创新中心河南省南水北调中线水源区生态安全重点实验室,河南南阳473000

出　　处：《中国油脂》2018年第5期104-109,共6页China Oils and Fats

基　　金：国家自然科学基金项目(31372381)

摘　　要：采用发酵液上清液进行小球藻培养试验研究。以小球藻的生物量为指标,通过上清液回用比例试验、单因素试验和L_9(3~4)正交试验进行配方优化,考察利用上清液培养小球藻的可行性。结果表明:上清液回用比例为30%(占配料用水量)处理效果最佳,在此基础上的配方优化结果为酵母粉2 g/L、K_2HPO_40.3 g/L、Mg SO_40.2 g/L、微量元素母液0.5 m L;在此工艺条件下,上清液首次回用小球藻在发酵末期的生物量比未添加上清液对照组高71%左右,二次、三次回用依次降低,但仍高于对照组,进一步验证了小球藻在优化配方下能够有效利用小球藻发酵液上清液促进自身生长,并且通过发酵液上清液的循环使用,可以节省30%的用水量、40%的营养物质(不含糖)用量并且可以减少环境污染。The experiment of chlorella vulgaris growing with supernatant in the fermentation broth was carried out. Taking the biomass of ehlorella vulgaris as the indexes, through the supernatant recycle ratio experiment, single factor experiment and L9 （34） orthogonal experiment to optimize formulation, the feasibility to grow the chloreUa vulgaris with supernatant was investigated. The results showed that the recycle ratio of supernatant accounted for 30% of the total fermentation broth with the best treatment effect. The optimal formulation was 2 g/L yeast powder, 0.3 g/L K2HPO4, 0.2 g/L MgSO4 and 0.5 mL microelement mother liquor. Under these technological conditions, the biomass of chlorella vulgaris in the end stage of fermenta- tion in the first time recycling of supernatant was about 71% higher than control group. The second and third time recycling were higher than control group even decrease in turn. It proved that the chlorella vulgaris could promote its growth with the optimal formulation supematant. The recycling of supernatan in the fermentation broth could save 30% of water consumption, 40% of the amount of nutrients and reduce environmental pollution.

关 键 词：小球藻 发酵液上清液 回用 配方 生物量 

分 类 号：TQ517.2[化学工程] Q934[生物学—微生物学]