天麻醇提物对灰树花胞外多糖单糖组成和生物活性的影响  

Effects of Rhizoma gastrodiae Alcohol Extract on Monosaccharide Composition and Biological Activity of Grifola frondosa Exopolysaccharide

作　　者：芦红云 吴天祥 汤庆莉[1] 钟敏 聂文强 LU Hongyun;WU Tianxiang;TANG Qingli;ZHONG Min;NIE Wenqiang(School of Liquor ＆ Food Engineering, Guizhou University, Guiyang 550025, China;Mingde College, Guizhou University, Guiyang 550025, China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学明德学院,贵州贵阳550025

出　　处：《食品科学技术学报》2018年第3期40-47,共8页Journal of Food Science and Technology

基　　金：国家自然科学基金地区科学基金项目(31460537).

摘　　要：天麻醇提物参与灰树花液体发酵体系,检测所得胞外多糖的单糖组成变化,研究天麻醇提物对灰树花胞外多糖生物活性的影响。利用高效液相色谱对4种胞外多糖样品进行单糖组成分析,采用小鼠巨噬细胞(RAW264.7)活化模型、人肝癌细胞(HepG 2)模型对灰树花胞外多糖生物活性进行检测。实验结果表明灰树花粗多糖样品主要由葡萄糖、甘露糖及少量的半乳糖等单糖构成;经纯化的胞外精多糖由甘露糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖、岩藻糖等单糖构成,单糖物质的量比依次为13.8∶3.9∶5∶3.7:1∶1.7,而添加天麻醇提物的胞外精多糖单糖组分物质的量比为12.7∶3.2∶5.6∶3.5∶1∶1.6。通过灰树花胞外多糖对小鼠巨噬细胞活化实验的结果可知,添加天麻醇提物的胞外精多糖对巨噬细胞有明显的活化作用,胞外精多糖、添加天麻醇提物制备的胞外粗多糖对巨噬细胞有一定的活化作用,胞外粗多糖对巨噬细胞无明确的活化作用。通过体外实验检测,4种多糖样品对人肝癌细胞HepG 2无抑制作用。添加天麻醇提物对灰树花胞外多糖单糖组分含量产生明显变化,但并未使灰树花多糖产生新单糖;添加天麻醇提物能提高灰树花胞外多糖对巨噬细胞的活化作用。The composition and anti-tumor bioactivity of the exopolysaccharide monosaccharide was carried out by adding Rhizoma gastrodiae alcohol extract to the liquid fermentation system of Grifola frondosa . The HPLC was applied to analyse the monosaccharide composition of exopolysaccharide samples and results showed that the crude exopolysaccharide samples were composed of glucose, mannose, and a small amount of galactose. Whereas the mannose, rhamnose, glucose, galactose, arabinose, fucose etc were the main monosaccharide composition of pure exopolysaccharides. Furthermore, the addition of Rhizoma gastrodiae alcohol extract had a significant change in the content of the monosaccharide component of the exopolysaccharide, but did not produce the new monosaccharide component of the polysaccharide. In the sample of pure exopolysaccharides, the monosaccharide molar ratio of mannose： rhamnose： glucose： galactose, arabinose： fucose was 13.8 ∶3.9 ∶5 ∶3.7 ∶1 ∶1.7, while the pure exopolysaccharides fermented with Rhizoma gastrodiae monosaccharide mole ratio was 12.7 ∶3.2 ∶5.6 ∶3.5 ∶1 ∶1.6. The results about of the biological activity Grifola frondosa exopolysaccharide on mice macrophage （RAW264.7） showed that the pure exopolysaccharides fermented with Rhizoma gastrodiae had obvious activation on macrophage. The pure exopolysaccharides and exopolysaccharides obtained by fermentation with Rhizoma gastrodiae had certain activation on macrophage. The activation of macrophages by crude exopolysaccha- rides was not significant. In vitro , four polysaccharide samples showed no inhibitory effect on human hepatoma HepG 2 cells.

关 键 词：天麻醇提物 灰树花 单糖组成 小鼠巨噬细胞RAW264.7 人肝癌细胞HepG 2 

分 类 号：TS201.3[轻工技术与工程—食品科学] TQ920.1[轻工技术与工程—食品科学与工程]