地塞米松诱导小鼠腭裂的母体血浆代谢组学分析  

Analysis of Metabonomics on Maternal Plasma in Mice with Cleft Palate Induced by Dexamethasone

作　　者：王世林 余子敬 何苇[1] WANG Shi--lin;YU Zi--jing;HE Wei .(Department of Oral and Maxillofacial Surgery, The Affiliated Stomatologieal Hospital of Zunyi Medical University, Zunyi 563000, China)

机构地区：[1]遵义医学院附属口腔医院口腔颌面外科,贵州遵义563000

出　　处：《口腔医学研究》2018年第5期485-489,共5页Journal of Oral Science Research

基　　金：贵州省科学技术基金项目(黔科合J字LKZ[2010]01号)

摘　　要：目的:应用代谢组学技术比较地塞米松干预后孕鼠与对照孕鼠在胚胎腭突发育期间的血浆代谢产物的差异,为发现地塞米松致畸时特征性代谢产物奠定一定基础。方法:将孕鼠随机分为地塞米松组(实验组)和生理盐水对照组,特定孕期分别给实验组和对照组小鼠腹腔注射一定量的地塞米松和生理盐水,并于胚胎17.5d收集孕鼠血浆样本并统计胎鼠腭裂发生率。应用核磁共振波谱仪获取孕鼠血浆样本的核磁共振氢谱图,结合主成分分析法和偏最小二乘法描绘实验组和对照组孕鼠血浆代谢产物在胚胎腭突发育阶段的代谢差异,获得地塞米松致腭裂的特异性代谢标志物。结果:实验组和对照组孕鼠在胚胎腭突发育期间血浆代谢产物有差异。结论:地塞米松可能通过引起胚胎腭突融合阶段氨基酸信号的改变,启动相关调控机制使胚胎发育延缓而导致腭裂的发生。Objective：To discover the difference of dexamethasone group and control group of pregnant mice in plasma metabolites during the embryonic palate development,and to lay foundations for discovering the specific metabolites during dexamethasone-induced cleft palate.Methods：Pregnant mice were collected and randomly divided into dexamethasone group（experimental group）and control group.During the specific pregnancy,the mice were peritoneally injected with certain amount of dexamethasone and saline respectively.After pregnant mice were sacrificed,plasma samples were collected in specific pregnancy and the incidence of mice cleft palate was estimated.Nuclear magnetic resonance spectrometer was applied to get the nuclear magnetic resonance hydrogen spectrum diagram of pregnant mice plasma samples.When combined with principal component analysis and partial least squares depict the metabolic differences between the experimental group and the control group of pregnant mice plasma metabolites in the development stage of embryonic palate,specific metabolic markers of cleft palate induced by dexamethasone were obtained.Results：The experimental group and the control group were different in plasma metabolites during embryonic palatal development.Conclusion：Dexamethasone may cause changes in amino acid signal during the embryonic palatal fusion phase,and activate the related regulation mechanism,which delayed the embryonic development and resulted in cleft palate.

关 键 词：地塞米松 腭裂 核磁共振氢谱 偏最小二乘法 

分 类 号：R782.2[医药卫生—口腔医学]