GATA6促进骨髓间充质干细胞成骨分化  

GATA6 Promotes Osteogenesis of Bone Marrow Mesenchymal Stem Cells

作　　者：孟凡彪 张树菊 金美玲 钱民先 刘佐君 MENG Fan-biao;ZHANG Shu-ju;JIN Mei-ling;QIAN Min-xian;LIU Zuo-jun(Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China)

机构地区：[1]深圳大学医学院,广东深圳518060

出　　处：《现代生物医学进展》2018年第8期1431-1434,1406,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81501206;81601215);深圳市科技研发基金项目(JCYJ20150525092941002)

摘　　要：目的:阐明锌指蛋白GATA6(GATA Binding Protein 6,GATA6)在骨髓间充质干细胞成骨分化作用。方法:利用基于慢病毒感染的方法建立高表达与敲低GATA6稳定的骨髓间充质干细胞系,并利用成骨诱导液进行成骨诱导,以茜素红S染色。用蛋白杂交方法检测RUNX2(Runt-related transcription factor 2,RUNX2)蛋白表达量,并利用免疫共沉淀的技术检测GATA6与RUNX2的相互作用。结果:数据表明GATA6高表达促进了骨髓间充质干细胞的成骨分化,并上调RUNX2蛋白表达量。在敲低GATA6后,RUNX2的表达量亦随之降低。免疫共沉淀检测进一步发现,GATA6与RUNX2具有相互作用。另外,GATA6在骨质疏松大鼠来源的骨髓间充质干细胞的表达量下降。结论:GATA6促进骨髓间充质干细胞成骨分化,为理解成骨作用机制提供了理论基础。Objective： The aim is to elucidate the role of zinc finger containing protein GATA6 in osteogenesis. Methods： GATA6 overexpressing and knock-down MSCs stable cell line were established and be induced for osteogenesis, which were detected by alizarin red S. The protein level of RUNX2 was detected by Western Blot, and the interaction of RUNX2 and GATA6 was examined by co-immunoprecipitation. Results： The data shows ectopic expression of GATA6 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells and upregulates the expression of RUNX2. The protein level of RUNX2 decrease after knocking down GATA6.Furthermore, GATA6 interacts with RUNX2 revealed by co-immunoprecipitation experiment. In addition, the protein level of GATA6 decreases in OVX-MSCs compared to wild type MSCs. Conclusions： GATA6 promotes the osteogenesis of bone marrow mesenchymal stem cells, providing a new understanding of the mechanism of osteogenesis.

关 键 词：GATA6 骨髓间充质干细胞 RUNX2 成骨分化 

分 类 号：R-33[医药卫生] R331.2