利用大豆油脱臭馏出物酶法合成植物甾醇酯  

Enzymatic synthesis of phytosterol ester from soybean oil deodorizer distillate

作　　者：林惠颖 辛嘉英[1,2] 么婷婷[1] 韩雪[1] 陈书明[3] LIN Hui-ying;XIN Jia-ying;YAO Ting-ting;HAN Xue;CHEN Shu-ming(Key Laboratory for Food Science and Engineering, Harbin University of Commerce,Harbin 150076, China;State Key Laboratory for Oxo Synthesis and Selective Oxidation ,Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801, China)

机构地区：[1]哈尔滨商业大学,食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]中国科学院,兰州化学物理研究所,羰基合成与选择氧化国家重点实验室,甘肃兰州730000 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《食品工业科技》2018年第11期62-66,共5页Science and Technology of Food Industry

基　　金：山西省优秀人才科技创新项目(201705D211029);山西省重点研发计划(指南)项目(201603D221027-3);哈尔滨商业大学创新团队项目(2016TD006).

摘　　要：从大豆油脱臭馏出物中分别分离得到植物甾醇和脂肪酸,对植物甾醇的分离提取条件进行优化,确定甲醇-丙酮混合溶剂的比例为1∶2(V/V),溶剂原料比为3∶1(V/W),洗涤粗甾醇的溶剂为正己烷,分离出的脂肪酸主要为油酸、亚油酸等不饱和脂肪酸。通过物理吸附法固定CRL脂肪酶,考察不同载体固定脂肪酶的担载率和酶活力,确定大孔树脂HP20为最佳固定载体,酶活力最高且担载率最大。分离得到的植物甾醇和脂肪酸在固定化酶的催化作用下酯化合成植物甾醇酯,酯化率为45.11%,为日后各领域的应用提供了新的思路。Phytosterol and fatty acid were isolated from soybean oil deodorizer distillate respectively.The extracting conditions of phytosterol were optimized and the optimal conditions were as follows ： the ratio of methanol to acetone was 1 ： 2 (V/V) ; the ratio of solvent to sample was 3：1 （V/W） ;n-hexane was selected as detergent.The isolated fatty acid mainly included oleic acid and linoleic acid.CRL lipase was immobilized by physical adsorption and immobilization degree and enzyme activity were determined as selecting indexes.HP20 resin was determined as the most suitable carrier because of its good performance.The synthesis of phytosterol fatty acid ester was carried out by the obtained phytosterol and fatty acid under the catalysis of immobilized enzyme, which provides a new pathway for the practice of all kinds of areas.

关 键 词：大豆油脱臭馏出物 植物甾醇 脂肪酸 

分 类 号：TS201.2[轻工技术与工程—食品科学]