QuEChERS-气相色谱法测定马铃薯中α-萘乙酸甲酯  

Determination of α-naphthaleneacetic acid methyl ester in potatoes by QuEChERS with gas chromatography

作　　者：刘仲义 黄秋研 霍丽斯 邱启东 陈卫强 LIU Zhong-Yi;HUANG Qiu-Yan;HUO Li-Si;QIU Qi-Dong;CHEN Wei-Qiang(Guangdong Testing Institute for Product Quality Supervision, Foshan 528300, Chin)

机构地区：[1]广东产品质量监督检验研究院,佛山528300

出　　处：《食品安全质量检测学报》2018年第10期2485-2488,共4页Journal of Food Safety and Quality

摘　　要：目的建立Qu ECh ERS-气相色谱法(gas chromatography,GC)测定马铃薯中α-萘乙酸甲酯含量的方法。方法样品经0.2%酸性乙腈和Qu ECh ERS盐包提取,旋蒸浓缩后用甲醇溶液复溶,经Qu ECh ERS分散固相萃取试剂盒净化,采用毛细管柱DB-5(30 m×0.25 mm,0.25μm)分离,火焰离子化检测器检测,外标法定量。结果在1~100 mg/L浓度范围内,α-萘乙酸甲酯具有良好的线性关系(r=0.9998),在0.5、2.5、5.0 mg/kg 3个浓度水平下,回收率范围为91.2%~96.7%,此方法的相对标准偏差(relative standard deviation,RSD)为0.75%~1.12%,方法检出限为0.05 mg/kg,定量限为0.2 mg/kg。结论此方法简便、快速、准确,可以用于马铃薯中α-萘乙酸甲酯含量的检测。Objective To establish a method for determination of α-naphthaleneacetic acid methyl ester in potatoes by QuEChERS with gas chromatography（GC）. Methods Samples were extracted by 0.2% acid acetonitrile and Qu ECHERS salt pack, concentrated by vortex evaporation and reconstituted by methanol, purified by Qu ECh ERS dispersive solid phase extraction kit, separated by capillary column DB-5（30 m×0.25 mm, 0.25 μm）, and then detected by flame ionization detector and quantified by external standard method. Results α-naphthaleneacetic acid methyl ester showed a good linearity in the range of 1-100 mg/L（r=0.9998）. The average recoveries were 91.2%-96.7% at the spiked levels of 0.5, 2.5, and 5.0 mg/kg with the relative standard deviations（RSDs） of 0.75%-1.12%. The limit of detection was 0.05 mg/kg and the limit of quantitative was 0.2 mg/kg. Conclusion This method is simple, rapid and accurate, which is suitable for determination of α-naphthaleneacetic acid methyl ester in potatoes.

关 键 词：Α-萘乙酸甲酯 马铃薯 气相色谱法 QuEChERS法 

分 类 号：O657.71[理学—分析化学]