羟丙基-β-环糊精对辣椒素的荧光增敏及其分析应用  

Fluorescence enhancement of hydroxypropyl-β-cyclodextrin on capsaicin and its analytical application

作　　者：黄铮 马永钧[1] 葛霞[3] HUANG Zheng;MA Yong-jun;GE Xia(College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070;Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agriculture Sciences, Lanzhou 730070;Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences, Lanzhou 730070)

机构地区：[1]西北师范大学化学化工学院,兰州730070 [2]甘肃省农业科学院畜草与绿色农业研究所,兰州730070 [3]甘肃省农业科学院农产品贮藏加工研究所,兰州730070

出　　处：《分析试验室》2018年第6期621-625,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(31660479);甘肃省自然科学基金项目(1606RJZA053);甘肃省青年科技基金项目(1506RJYA193)资助

摘　　要：采用荧光光谱法、等摩尔连续变化法(Job's plot)和相溶解度法考察了羟丙基-β-环糊精(HPβCD)与辣椒素主客体包合物的形成。结果表明,辣椒素可进入HPβCD的空腔而形成主客体比为1∶2的包合物,并且通过相溶解度图得到包合稳定常数Kc为743.95 L/mol。基于HPβCD对辣椒素荧光增强作用,建立了一种荧光光谱测定辣椒素的新方法。此外,通过体系酸度、HPβCD浓度、试剂的加入顺序和反应时间等对体系荧光强度的影响,优化了分析测试条件。辣椒素在0.05~20μg/mL范围内与荧光强度呈良好的线性关系,检出限为0.04μg/mL,相对标准偏差(RSD)为1.3%,在干辣椒样品中的回收率为99.5%~105.6%。The host-guest inclusion system of hydroxypropyl-β-cyclodextrin（HPβCD） with capsaicin was investigated by fluorescence spectra,Job＇s plot and phase solubility studies.The results showed that the capsaicin was encapsulated in the HPβCD＇s cavity to form a 1： 2 stoichiometry host-guest inclusion complex and the inclusion constant（Kc = 743.95 L/mol） was confirmed by the phase solubility diagram.Furthermore,several experimental conditions were optimized in order to obtain the maximum fluorescence signal.On the basis of the enhancement of the fluorescence of capsaicin produced through complex formation,a spectrofluorimetric method for the determination of capsaicin in bulk aqueous solution in the presence of HPβCD was developed.The linear relationship between the fluorescence intensity and capsaicin concentration was obtained in the range of 0.05-20 μg/m L.The detection limit was 0.04 μg/m L,and the relative standard deviation was 1.3%.The proposed method has been successfully applied to the determination of capsaicin in chili powder with recoveries of 99.5%-105.6%.

关 键 词：羟丙基-Β-环糊精 辣椒素 荧光分光光度法 包合物 

分 类 号：TS207.3[轻工技术与工程—食品科学]