淡水黑鲷及其与鲈亚目主要淡水养殖鱼类系统进化关系分析  

The complete mtDNA of sooty grunter （Hephaestusfuliginosus） and phylogenetic analysis of 19 species in the suborder Percoidei

作　　者：赵立祥[1,2] 董浚键 孙成飞[1,2] 田园园[1] 胡婕 叶星[1,2] ZHAO Llxaang;DONG Junjian;SUN HU Jie;YE Xing;Chengfe;TIAN Yuanyuan(Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510385;College of Fisheries and Life Ssience, Shanghai Ocean University, Shanghai 201306)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用鱼养殖重点实验,广州510385 [2]上海海洋大学,水产与生命学院,上海201306

出　　处：《珠江水产科学》2018年第2期44-67,共24页Finsheries Science and Technology Industry

基　　金：国家现代农业产业技术体系（CARS-46）;广东省科技计划项目（2015A020209034）;广东省海洋与渔业科技推广专项（A201601A06）;广东省科技计划项目（2015A020209034）;广东省自然科学基金-博士启动基金（2015A030310154）;中国水产科学研究院基本科研业务费资助（2017HY-XKQ0208）

摘　　要：鲈形目是鱼类乃至脊椎动物中最大的目，中国大部分海产经济鱼类和多种重要的淡水养殖鱼类均属于其中的鲈亚目。淡水黑鲷是鲈亚目俐科的一种经济鱼类，为分析其与鲈亚目淡水主要养殖鱼类的系统进化关系，该研究扩增了淡水黑鲷线粒体基因组全序列，并从GenBank上检索到鲈亚目劁科、诣科与太阳鱼科共19种鱼类的线粒体基因组信息。其线粒体基因组总长为16770bp包括13个蛋白、2个rRNA与22个tRNA编码基因及一个非编码控制区D-loop，基因排列次序与分布与绝大部分脊椎动物的相同。通过邻接法等3种方法对基因组及COI、12SrRNA等6个单基因序列进行进化分析。进化树显示，蜊科、太阳鱼科各分聚一支，错科鱼类分聚两支；三种淡水劁科鱼类起源于海水鱼类且淡水黑鲷与宝石鲈的亲缘关系更近；并支持大口黑鲈两个亚种应是2个独立的种，鳜属鱼类应为独立的科。研究结果可为淡水黑鲷种质资源研究及远缘杂交育种提供分子基础。Perciformes is the largest order of fishes, and most economic marine fishes and freshwater fishes cultured in China belong to the Pereoidei suborder within Perciformes. Sooty grunter （Hephaestus fuliginosus） is an economic fish species in the Terapontidae family of Percoidei. To analyze the phylogenetie relationships between sooty grunter and major freshwater fishes in Percoidei, this study analyzed the whole sooty grunter mitochondrial genome and obtained the mitochondrial genome of 19 fishes from the Terapontidae, Serranidae, and Centrarchidae families in Percoidei from GenBank. The mitochondrial genome of sooty grunter was 16770 bp, encoding 13 proteins, two rRNAs, 22 tRNAs, and a non-encoded control region （D-loop）. The sequence and distribution of mitochondrial-eneoded genes were similar to those of most vertebrates. Phylogenetic analyses were conduct using the complete mitochondrial genome and six genes, including cytoehrome oxidase I, cytochrome B, 12S rRNA, ND2, ND4, and NDS, and the results of these analyses were consistent. In these phylogenetic trees, fishes in Terapontidae and Centrarchidae formed monophyletie clades, while fishes in Serranidae were divided into two. For the three freshwater fish in Terapontidae, the sooty grunter showed more closely relationship to jade perch. The results also suggested that freshwater Terapontidae fishes originate from marine fishes. In addition, Micropterus salmoides salmoides and Micropterus salmoidesfloridanus in Centrarehidae should be designated as two independent species, and Siniperca in Serranidae should be an independent family. The results would benefit the study of sooty grunter germplasm resources, and could provide molecular basis for cross-breeding of the cultured species of Percoidei.

关 键 词：淡水黑鲷 鲈亚目 系统进化分析 全线粒体基因组序列 单基因 

分 类 号：S943.1[农业科学—水产养殖]