美洛昔康在抑制肝癌SMMC-7721细胞侵袭和迁移的作用  

Effect of Meloxicam on invasion and migration of liver cancer SMMC - 7721 cellS and itsmecha-nism

作　　者：郑春雷[1] 王玉龙[1] 赵晶莹[1] 鲍红光[1] 孟庆辉[2] Zheng Chunlei;Wang Yulong;Zhao Jingying;Bao Hongguang;Meng Qinghui(Department of Tumor Surgery, the Second Affiliated Hospital of Qiqihar Medical, Qiqihar 161005, China;Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150081, Chin)

机构地区：[1]齐齐哈尔医学院附属第二医院肿瘤外科,161006 [2]哈尔滨医科大学附属第一医院普外科,150081

出　　处：《中华实验外科杂志》2018年第6期1052-1054,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察美洛昔康（Mel）在抑制肝癌SMMC-7721细胞侵袭和迁移的作用的影响及其机制。方法用锥虫蓝法检测不同浓度Mel对HL-7702人正常肝细胞的毒性作用；用（0、50、100、200）p,mol／mlMel处理肝癌SMMC-7721细胞，Transwell^TM实验检测肝癌SMMC-7721细胞的侵袭力；细胞划痕实验检测肝癌SMMC-7721细胞迁移力；Westernblot法测定肝癌SMMC-7721细胞环氧合酶-2（COX-2）、上皮型钙黏素（E-cadherin）、神经型钙黏素（N-cadherin）、基质金属蛋h酶（MMP）-2的蛋白水平。结果（0、50、100、200）μmol／mlMel对HL-7702人正常肝细胞无明显毒性作用，400μmol／mlMel毒性作用明显（P=0．007）。Mel以剂量相关方式抑制肝癌SMMC-7721细胞的侵袭和迁移能力；Westernblot结果显示Mel作用肝癌SMMC-7721细胞后，COX-2和N-cadherin蛋门表达降低，E-cadherin蛋白表达升高，而MMP-2蛋白的表达差异无统计学意义（P=0．128）。结论Mel在一定范围内对正常肝细胞不良反应小，可以抑制肝癌SMMC-7721细胞的侵袭和迁移，其机制之一肯能是通过调控COX-2及其下游通路影响了N-cadherin及E-cadherin的表达，从而达到了抑制上皮一问充质转化（EMT）。Objective To explore the effect and mecMnism of Meloxicam on migration and inva- sion of SMMC -7721 cells. Methods Trypan blue dye exclusion assay was used to determine the cytotox- ic effect of varying dosages of Mel on the HL - 7702 normal human hepaticcells. The migration and inva- sion of SMMC - 7721 cells were determined by wound scratch assay and TranswcllTM assay respectively. The expression of cyclooxygenase - 2 （ COX - 2） , E - cadherin, N - cadherin, matrix metalloproteinase （MMP） -2 in SMMC -7721 cells treated by Mel were detected by Western blotting. Results 0 - 200 μmoL/ml Mel were not significantly toxic to HL -7702 normal human hepaticcells, 400 p^moL/ml Mel had significant cytotoxic to HL - 7702 normal human hepaticcells （ P = 0. 007 ）. the abilities of invasion and migration of SMMC-7721 cells were inhibited by Mel. The expression of COX -2 and N -cadherin were decreased but E- cadherin increased and MMP -2 had no significant difference after Mel treatment （P = 0. 128 ）. Conclusion Mel is less toxic to normal cells, also can inhibit the abilities of proliferation, migration and invasion of SMMC -7721 cells that might be related to regulate the expression of COX -2, N -cadherin and E - cadherin. Thus, the transformation process of epithelial - mesencbymal transition （EMT）.

关 键 词：美洛昔康 SMMC-7721细胞 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]