长链非编码RNANR2F2-AS1在人上皮性卵巢癌中的表达  

Expression of long non-coding RNA NR2F2-AS1 in human epithelial ovarian cancer

作　　者：尼华[1] 牛蕾蕾[1] 景兰凯 张乐天 杜权[2] 李华[1] NI Hua;NIU Leilei;JING Lankai;ZHANG Letian;DU Quan;LI Hua(Department of Gynecology and Obstetrics, Peking University Third Hospital, Beijing 100083, China;State Key Laboratory of Natural and Biomimetic Drugs, Peking University Heahh Science Center, Beijing 100083, China)

机构地区：[1]北京大学第三医院妇产科,北京100083 [2]北京大学医学部天然药物与仿生药物国家重点实验室,北京100083

出　　处：《肿瘤》2018年第6期581-589,共9页Tumor

基　　金：国家自然科学基金自助项目(编号:81572765)~~

摘　　要：目的:分析长链非编码RNA(long non-coding RNA,lnc RNA)NR2F2(chicken ovalbumin upstream promoter-transcription factorⅡ,又称nuclear receptor subfamily 2 group F member 2)反义核糖核酸1(NR2F2-antisense RNA 1,NR2F2-AS1)在人上皮性卵巢癌中的表达。方法:利用Aligent Human lncRNA基因芯片检测3对卵巢癌组织及其对应癌旁组织中lnc RNA和mRNA的表达,筛选出差异表达的lnc RNA和mRNA。应用实时荧光定量PCR检测22例卵巢癌组织和10例良性卵巢囊肿组织中lncRNA NR2F2-AS1、核仁小RNA宿主基因4(small nucleolar RNA host gene 4,SNHG4)、LOC101927905和OVE5-21006的表达。将靶向NR2F2-AS1的siRNA转染卵巢癌SKOV3细胞后,应用实时荧光定量PCR检测NR2F2-AS1反义基因NR2F2的表达。结果:3对卵巢癌组织及其对应癌旁组织的基因芯片检测共筛选出33 403条差异表达的lnc RNA和20 351条差异表达的mRNA。卵巢癌组织中NR2F2-AS1的表达水平明显低于良性卵巢囊肿组织(P=0.003 5)。并且,Ⅰ～Ⅱ期卵巢癌组织中NR2F2-AS1的表达水平低于Ⅲ～Ⅳ期卵巢癌组织(P=0.0422)。靶向NR2F2-AS1的siRNA转染SKOV3细胞后,NR2F2 mRNA的表达水平被明显下调(P=0.049 5)。结论:上皮性卵巢癌组织中lncRNA表达谱与相应癌旁组织有较大差异。NR2F2-AS1在卵巢癌组织中表达下调,并且与卵巢癌患者的病理分期有关。抑制卵巢癌SKOV3细胞中NR2F2-AS1的表达后,其反义基因NR2F2的表达水平也下降。Objective： To analyze the expression of long non-coding RNA （IncRNA） nuclear receptor subfamily 2 group F member 2 （NR2F2）-antisense RNA 1 （AS1） in human epithelial ovarian cancer.Methods： Agilent human IncRNA microarray was used to detect the expressions of IncRNA and mRNA in 3 pairs of ovarian cancer tissues and para-cancerous tissues. The abnormally expressions of mRNAs and IncRNAs were screened. Real-time fluorescent quantitative PCR was performed to detect the expression levels of IncRNA NR2F2-AS1, small nucleolar RNA host gene 4 （SNHG4）, LOC101927905 and OVE5-21006 in 22 ovarian cancer tissues and 10 benign ovarian cyst tissues. Moreover, the expression of NR2F2 （an antisense gene of NR2F2-AS1） in ovarian cancer SKOV3 cells after transfection with the specific siRNA targeting NR2F2-AS1 was detected by real-time fluorescent quantitative PCR.Results： Based on microarray analysis, a total of 33 403 IncRNAs and 20 351 mRNAs with different expression levels were screened from 3 pairs of epithelial ovarian cancer and paracancerous tissues. The expression level of NR2F2-ASl in ovarian cancer tissues was lower than that in benign ovarian cyst tissues （P = 0.003 5）. The expression level of NR2F2-ASI in patients with ovarian cancer of stage Ⅰ - Ⅱ was lower than that of stage Ⅲ-Ⅳ （P = 0.042 2）. After the transfection with NR2F2-ASl siRNA, the expression level of NR2F2 mRNA was downregulated in SKOV3 cells （P = 0.049 5）.Conclusion： The expression profiles of IncRNAs in ovarian cancer tissues are significantly different from those in the corresponding para-cancerous tissues. NR2F2-AS1 is downregulated in ovarian cancer tissues, and is related to the pathological stage of ovarian cancer. After inhibiting the expression of NR2F2-AS1 in ovarian cancer SKOV3 cells, the expression level of its antisense gene NR2F2 is decreased.

关 键 词：卵巢肿瘤 RNA 长链非编码 微阵列分析 COUP转录因子Ⅱ 

分 类 号：R737.31[医药卫生—肿瘤]