灰树花液态发酵菌丝体工艺优化及其体外抗氧化活性  

Optimization of Submerged Fermentation in Mycelia by Grifola frondosa and Its Antioxidant Activities

作　　者：吴林秀[1,2] 胡荣康 雷雅婷[2,3] 陈永芳 林国鹏 陈岚彬 陈敏 刘斌 WU Lin-xiu;HU Rong-kang;LEI Ya-ting;CHEN Yong-fang;LIN Guo- peng;CHEN Lan- Bin;CHEN Min;LIU Bin(College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;National Engineering Research Center of JUNCAO Technology, Fuzhou 350002, China;College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002 [3]福建农林大学生命科学学院,福建福州350002

出　　处：《食品工业科技》2018年第13期187-191,197,共6页Science and Technology of Food Industry

基　　金：国家科技支撑计划子课题(2014BAD15B01);福建省科技重大专项(2014NZ2002-1);福建省科技厅区域发展项目(2016N3006)

摘　　要：以葡萄糖、蛋白胨、KH_2PO_4、果蔬汁用量为影响因素,应用响应面分析法探讨灰树花液体发酵的最优条件,并以菌丝体生物量为指标,测定体外抗氧化活性。实验结果表明,100 m L液体培养基,接种量10%时,最优培养基的配方:葡萄糖2.5%(w·v^(-1)),蛋白胨0.5%(w·v^(-1)),KH_2PO_40.1%(w·v^(-1)),果蔬汁15%(v·v^(-1))。在此条件下菌丝体生物量预测值为19.06 mg·m L^(-1),与实际测量值(18.12±0.214)mg·m L^(-1)在误差范围内。抗氧化结果表明,灰树花菌丝体具有较强的清除能力,当质量浓度为1.5 mg·m L^(-1)时,DPPH自由基的清除率达到69.78%±6.17%;ABTS自由基的清除率达到82.11%±7.24%;羟基自由基清除率达到59.73%±3.15%。因此,灰树花液体发酵培养的菌丝体可以作为潜在的抗氧化剂。In this paper, the optimal conditions for the fermentation of Grifola frondosa were studied by response surface methodology with glucose, peptone, KH2PO4 , fruit and vegetable jujce dosage as factors and mycelia biomass as the index, and the antioxidant activity of mycelia was measured. The results showed that the optimal formula was： glucose dosage 2.5 % （ w·v-1）, peptone dosage 0.5 % （ w·v-1 ） , KH2 PO4 dosage 0.1 fro （ w·v-1 ） , fruit and vegetable juice dosage 15 % （ w·v-1 ）. In this condition, the mycelial dry weight prediction value was 19.06 mg·mL-1, and the actual measured value is （18.12 ± 0.214） mg·mL-1 within the error range.The results showed that the scavenging rate of DPPH radical was 69.78% ± 6.17% when the mass concentration was 1.5 mg·mL-1,and the clearance rate of ABTS free radical was 82.11% ±7.24% ;Hydroxide radical scavenging rate reached 59.73 % -3.15 % .Thus, the mycelia of the submerged fermentation of Grifola frondosa could be used as a potential antioxidant.

关 键 词：灰树花 液体发酵 菌丝体 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]