蛹虫草黄色素提取工艺优化及其体外抗氧化研究  

Extraction and anti-oxidative activity of yellow pigment from Cordyceps militaris

作　　者：张明亮 刘靖宇[1,2] 常明昌 孟俊龙[1,2] 赵慧 侯笑笑[1] ZHANG Ming-Liang;LIU Jing-Yu;CHANG Ming-Chang;MENG Jun-Long;ZHAO Hui;HOU Xiao-Xiao(College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030802, China;Collaborative Innovation Center of Quality and Efficiency of Loess Plateau Edible Fungi in Shanxi, Shanxi Agricultural University, Taigu, Shanxi 030802, China)

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]黄土高原食用菌提质增效协同创新中心,山西太谷030801

出　　处：《菌物学报》2018年第6期761-771,共11页Mycosystema

基　　金：山西省煤基重点科技攻关项目(FT2014-03-01);山西省重点研发计划重点项目(201603D21106)~~

摘　　要：本研究为了得到蛹虫草黄色素最佳提取工艺,采用Box-Behnken方法,在单因素试验的基础上以黄色素的提取率为响应值,利用响应面设计对超声波辅助乙醇提取蛹虫草黄色素工艺进行优化,并对其体外抗氧化活性进行了研究。结果表明超声波辅助乙醇提取蛹虫草黄色素的最佳工艺条件为乙醇体积分数57.00%、液料比32.00:1、提取时间18.00min,在此条件下黄色素提取率达(1.976±0.017)mg/g。红外光谱和高效液相色谱的结果表明,该提取条件下获得的蛹虫草黄色素主要成分为cordyxanthin。蛹虫草黄色素DPPH自由基清除力IC50为0.59mg/m L,在5mg/m L时,总抗氧化力高达0.587,在10mg/m L时,铁离子还原力高达1.488。This study aims at obtaining optimum ultrasonic-assisted ethanol extraction process for yellow pigment from Cordyceps militaris（YPCM）. The response surface methodology with Box-Behnken test was designed on the basis of the single-factor designs. The antioxidant activities of YPCM were systematically investigated in vitro. The results showed that the optimal extraction conditions of YPCM were ethanol concentration of 57.00%, solvent-to-solid ratio of 32.00：1 and extraction period of 18.00 min. Under these conditions the extraction yield of YPCM was（1.976±0.017）mg/g. The data of IR and HPLC showed that YPCM was composed of a set of cordyxanthin. Analysis of the antioxidant activities of YPCM showed the DPPH free radical scavenging force IC50 was 0.59 mg/m L, and the total antioxidant power reached 0.587 when the mass fraction of YPCM was 5 mg/mL, and the iron ion reduction force reached 1.488 when the mass fraction of YPCM was 10 mg/mL.

关 键 词：蛹虫草 黄色素 超声波 响应面 抗氧化 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]