PD-L1下调对口腔鳞癌细胞生物学行为的影响  

Effect of PD-L1 downregulation on biological properties in oral squamous cell carcinoma

作　　者：王晓宁[1] 秦星[1] 王旭[1] 陈万涛[1] WANG Xiao-ning;QIN Xing;WANG Xu;CHEN Wan-tao.(Department of Oral and Maxillofacial-Head and Neck Oncology , College of Stomatology , Ninth People＇ s Hospital, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology ＆ Shanghai Research Institute of Stomatology, Shanghai 200011, China)

机构地区：[1]上海交通大学医学院附属第九人民医院及口腔医学院口腔颌面-头颈肿瘤科,国家口腔疾病临床研究中心,上海市口腔医学研究所及上海市口腔医学重点实验室,上海200011

出　　处：《北京口腔医学》2018年第3期127-131,共5页Beijing Journal of Stomatology

基　　金：国家重点研发计划(2016YFC0902703);上海市科学技术委员会项目(16431903300)

摘　　要：目的研究PD-L1基因对口腔鳞癌细胞生物学行为的影响,初步探讨PD-L1在口腔鳞癌进展过程中发挥的作用。方法采用脂质体2000(lipofectamine 2000)转染小干扰RNA(small interference RNA,siRNA)的方法,对口腔鳞癌细胞系细胞中PD-L1基因进行敲减,real-time PCR检测敲减效果。利用MTT法比较PD-L1敲减组与对照细胞增殖能力的差异。克隆形成实验评价PD-L1对肿瘤细胞克隆形成能力的影响。划痕实验研究PD-L1对细胞迁移能力的影响。Transwell实验验证PD-L1对细胞侵袭能力的影响。提取口腔鳞癌病人组织标本RNA并通过real-time PCR的方法比较PD-L1的表达差异。结果转染siRNA后,细胞PD-L1表达明显下降,PD-L1敲减细胞的增殖、克隆形成、迁移和侵袭能力都较对照组细胞减弱。在临床标本的检测中,证实了PD-L1在口腔鳞癌组织中高表达。结论 PD-L1能对口腔鳞癌细胞的增殖、克隆形成、迁移及侵袭能力都具有明显影响,可能在口腔鳞癌的进展过程中发挥一定作用。Objective To investigate the effect of PD-L1 on the biological properties of oral squamous cell carcinoma（ OSCC） cells,and the possible function of PD-L1 in the progression of OSCC. Methods RNA interference oligonucleotides were transfected with Lipofectamine 2000,real-time PCR was performed to evaluate the efficiency of gene knock-down. The MTT assay,clonogenicity assay,wound healing assay and transwell assay were utilized to investigate the functions of PD-L1 in biological characteristics of OSCC,including proliferation,migration and invasion. Besides,realtime PCR was implemented to detect the PD-L1 expression in OSCC samples. Results PD-L1 was knocked down in OSCC cell lines by siRNA. The down-regulation of PD-L1 attenuated the proliferation,colony formation,migration and invasion of OSCC cells. The overexpression of PD-L1 was verified in OSCC tissues compared to the adjacent normal ones. Conclusion PD-L1 may have effect on proliferation,migration and invasion of OSCC cells and play a role in the development of OSCC.

关 键 词：PD-L1 口腔鳞癌 增殖 迁移 

分 类 号：R739.8[医药卫生—肿瘤]