Synthesis of Ras proteins and their application in biofunctional studies  

作　　者：Jun Hu Pengcheng Zhu Yanmei Li Yongxiang Chen 

机构地区：[1]Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education),Department of Chemistry,Tsinghua University

出　　处：《Chinese Chemical Letters》2018年第7期1043-1050,共8页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China (Nos. 21672125, 91753122)

摘　　要：Approximately 30% of human cancers are associated with RAS mutation. Ras proteins on the plasma membrane regulate a plenty of important cellular processes. The post-translational modifications(PTMs)of Ras proteins like lipidation and methylation are crucial for their correct cellular localization and biological function. Hence, obtaining Ras proteins with different kinds of modifications is the necessary prerequisite to investigate their biological properties at molecular level. In this review, we mainly summarize the developed strategies including chemical total synthesis, biosynthesis and semi-synthesis for producing Ras proteins with modifications and their application in biological studies.Approximately 30% of human cancers are associated with RAS mutation. Ras proteins on the plasma membrane regulate a plenty of important cellular processes. The post-translational modifications(PTMs)of Ras proteins like lipidation and methylation are crucial for their correct cellular localization and biological function. Hence, obtaining Ras proteins with different kinds of modifications is the necessary prerequisite to investigate their biological properties at molecular level. In this review, we mainly summarize the developed strategies including chemical total synthesis, biosynthesis and semi-synthesis for producing Ras proteins with modifications and their application in biological studies.

关 键 词：RAS LIPOPEPTIDES SEMI-SYNTHESIS Multi-PTMs Biophysical function 

分 类 号：O629.73[理学—有机化学]