Preliminary investigation on cytotoxicity of fluorinated polymer nanoparticles  

作　　者：Xue Wang Wenge Cheng Qiuyuan Yang Hongyun Niu Qian Liu Yun Liu Ming Gao Ming Xu An Xu Sijin Liu Xiaoyu Huang Yuguo Du 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy ofSciences, Beijing 100085, China [2]School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China [3]University of Chinese Academy of Sciences, Beijing 200049, China [4]School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, China [5]Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Anhui Province, Hefei230031, China [6]Key Laboratory of Organofluorine Chemistry and Laboratory of Polymer Materials, Shanghai Institute of Organic Chemistry, ChineseAcademy of Sciences, Shanghai 200032, China

出　　处：《Journal of Environmental Sciences》2018年第7期217-226,共10页环境科学学报（英文版）

基　　金：supported by grants under the National “973” program (No. 2015CB931903);grants from the National NaturalScience Foundation of China (No. 21407169)

摘　　要：As well-known persistent organic pollutants（POPs）, organofluorine pollutants such as perfluorooctane sulfonate（PFOS） have been proven to be bioaccumulated and harmful to health. However, toxicological assessment of organofluorinated nanoparticles, which have emerged as a novel tool for biomedical and industrial applications, is lacking, to the best of our knowledge. To assess the biological effects and health risk of fluorinated nanoparticles,trifluoroethyl aryl ether-based fluorinated poly（methyl methacrylate） nanoparticles（PTFEPMMA NPs） were synthesized with various fluorine contents（PTFE-PMMA-1 NPs 12.0 wt.%,PTFE-PMMA-2 NPs 6.1 wt.% and PTFE-PMMA-3 NPs 5.0 wt.%）, and their cytotoxicity was investigated in this study. The in vitro experimental results indicated that the cytotoxicity of PTFE-PMMA NPs was mild, and was closely related to their fluorine（F） contents and Fcontaining side chains. Specifically, the cytotoxicity of PTFE-PMMA NPs decreased with increasing F content and F-containing side chains. After exposure to PTFE-PMMA NPs at a sublethal dose（50 μg/m L） for 24 hr, the phospholipid bilayer was damaged, accompanied by increasing permeability of the cell membrane. Meanwhile, the intracellular accumulation of reactive oxygen species（ROS） occurred, resulting in the increase of DNA damage, cell cycle arrest and cell death. Overall, the PTFE-PMMA NPs were found to be relatively safe compared with typical engineered nanomaterials（ENMs）, such as silver nanoparticles and graphene oxide, for biomedical and industrial applications.As well-known persistent organic pollutants（POPs）, organofluorine pollutants such as perfluorooctane sulfonate（PFOS） have been proven to be bioaccumulated and harmful to health. However, toxicological assessment of organofluorinated nanoparticles, which have emerged as a novel tool for biomedical and industrial applications, is lacking, to the best of our knowledge. To assess the biological effects and health risk of fluorinated nanoparticles,trifluoroethyl aryl ether-based fluorinated poly（methyl methacrylate） nanoparticles（PTFEPMMA NPs） were synthesized with various fluorine contents（PTFE-PMMA-1 NPs 12.0 wt.%,PTFE-PMMA-2 NPs 6.1 wt.% and PTFE-PMMA-3 NPs 5.0 wt.%）, and their cytotoxicity was investigated in this study. The in vitro experimental results indicated that the cytotoxicity of PTFE-PMMA NPs was mild, and was closely related to their fluorine（F） contents and Fcontaining side chains. Specifically, the cytotoxicity of PTFE-PMMA NPs decreased with increasing F content and F-containing side chains. After exposure to PTFE-PMMA NPs at a sublethal dose（50 μg/m L） for 24 hr, the phospholipid bilayer was damaged, accompanied by increasing permeability of the cell membrane. Meanwhile, the intracellular accumulation of reactive oxygen species（ROS） occurred, resulting in the increase of DNA damage, cell cycle arrest and cell death. Overall, the PTFE-PMMA NPs were found to be relatively safe compared with typical engineered nanomaterials（ENMs）, such as silver nanoparticles and graphene oxide, for biomedical and industrial applications.

关 键 词：Fluorinated nanoparticles POLYMER CYTOTOXICITY Oxidative stress Health risk 

分 类 号：X171.5[环境科学与工程—环境科学] X592