GC-MS联用法分析明党参及其快熟繁殖体系的化学成分  

Analysis of Chemical Constituents of Mingdangshen(Changii Radix) and Its Rapid Propagation System by Gas Chromatography-Mass Spectrometry

作　　者：姚晓 步达[2] 陈建伟[3] 李祥[4] YAO Xiao;BU Da;CHEN Jianwei;LI Xiang(Changzhou Food and Drug Administration,Changzhou 213002,China;College of Pharmaey,Nanjing Uni- versity of Chinese Medieine,Nanjing 210029,China;Jiangsu Key Laboratory for Traditional Chinese Formu- lae Research,Nanjing 210023,China;Jiangsu Key Laboratory for Chinese Material Mediea Processing,Nan- jing 210029, China)

机构地区：[1]常州市食品药品监督检验中心,江苏常州213002 [2]南京中医药大学药学院,江苏南京210029 [3]江苏省方剂研究重点实验室,江苏南京210023 [4]江苏省中药炮制重点实验室,江苏南京210029

出　　处：《山东中医药大学学报》2018年第4期358-362,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：高等学校博士学科点专项科研基金资助课题(编号:200803150009);国家药典委员会(编号:YZ-124,YZ-125);江苏省自然科学基金(编号:BK2003107)

摘　　要：采用乙醚超声提取法分别对栽培明党参根、明党参诱导根、明党参愈伤组织、炮制明党参根、野生明党参根进行提取,并用气相色谱-质谱对样品进行分析。从5个样品中共检出20种挥发性成分,以芳香族化合物和醇类为主。在检出的挥发性物质中,邻苯二甲酸二丁酯和丁基羟基甲苯是5个样品共有的。其中明党参愈伤组织成分较少,仅检测出4种成分;愈伤组织经诱导形成根后成分明显增多,检出8种成分,多于野生明党参（5种成分）,少于栽培明党参（13种成分）。栽培明党参不但满足植物生长发育和品质形成的要求,而且最大限度地提高了品质。同时组织培养适于明党参的快速繁殖且不受地域限制,并能通过对愈伤组织进行诱导生根,从而保证药材的质量。组织培养和栽培均为良种选育的重要途径。Ether ultrasonic extraction was used to extract the roots of cultivated Mingdangshen （Changu radix） ,induced roots, callus, prepared roots of Mingdangshen （Changii radix） and roots of wild Mingdangshen callus was induced to form the root,8 components were detected,more than the wild species of Mingdang shen（Changii radix）（5 components）,which was less than that of the cultivated plant. Cultivated Mingdangshen （Changii radix） can not only satisfy tile requirements for plant growth and quality formation,but also maxi- mizes quality. At the same time,tissue culture is suitable for the rapid propagation of cultivated Mingdang- shen（Changii Radix） and is not restricted by geographical regions. By inducing callus to form the roots,it ensures the quality of medicinal materials. Tissue culture and cultivation are important approaches for selec-tive breeding.

关 键 词：明党参 愈伤组织 诱导根 挥发性成分 GC-MS 

分 类 号：R284.1[医药卫生—中药学]