ARMS法与直接测序法检测非小细胞肺癌组织EGFR基因突变的结果对比分析  

Comparing analysisARMS and sequencing in detecting 127 NSCLC EGFR mutation

作　　者：李慧[1] 王秋实 李增鹏[2] 陈维贤[1] Li Hui;Wang Oiushi;Li Zengpeng;Chen Weixian(Clinical Laboratory of the Second Hospital of Chongqing Medical University,Chongqing,400000,China;Department of Pathology of Daping Hospital,Chongqing,400042,China)

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400000 [2]第三军医大学大坪医院病理科,重庆400042

出　　处：《医药前沿》2018年第18期10-12,共3页Journal of Frontiers of Medicine

摘　　要：目的：通过两种方法，即直接测序法与扩增突变阻滞系统（ARMS）法，对表皮生长因子受体（EGFR）基因突变检测进行对比。方法：收集2012年7月-2013年11月在第三军医大学附属大坪医院病理科通过病理标本活检确诊为非小细胞肺癌的患者127例，采用ARMS法和直接测序法检测该人群EGFR基因的突变情况。结果综合两种方法，最终确定有突变的患者为64例，ARMS法检出60例，敏感性为93．75S，直接测序法检出33例，敏感性为51．56％。ARMS法的检测突变率为47．2％，而直接测序法检测突变率为26．0％．两组对比P〈0．05差异具有统计学意义。结论：ARMS法的突变检出率高于测序法，更适合临床应用．Objective This article aims to explore the accurate ,sensitive and convenient method for clinical use in China by compairing direct sequencing and amplification refractory mutation （ARMS）, helping to screen outa proper trerapeutic regimen for people. Methods ARMS and Sequencing Results Soothe two detection metheds are to some extent coincident（K=0.53,P〈0.05）. Integrating detection results by two metheds, there are 64 cases identified as mutated EGFR.The sensitivity of ARMS is 93.75%,reas the sensitivity of direct sequencing is 51.56%. The mutation detection rate of ARMS is significantiy higher than that of direct sequencing （47.2% vs 26.0%,P〈0.05）. Conclusion ARMS methed is more sensitive and higher mutantion rate.

关 键 词：非小细胞肺癌 表皮生长因子 基因突变 测序法 扩增突变阻滞系统 

分 类 号：R730.4[医药卫生—肿瘤]