QuEChERS EMR-Liqid-超高效液相色谱-串联质谱法测定动物源食品中的安眠酮  

Determination of methaqualone residues in animal-derived food by QuEChERS EMR-Liqid-ultra performance liquid chromatography-tandem mass spectrometry

作　　者：唐星畅 林立毅[1] 吴敏[1] 严丽娟[1] 林建忠[1] TANG Xing-Chang;LIN Li-Yi;WU Min;YAN Li-Juan;LIN Jian-Zhong(Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China)

机构地区：[1]厦门出入境检验检疫局,厦门361026

出　　处：《食品安全质量检测学报》2018年第13期3251-3257,共7页Journal of Food Safety and Quality

基　　金："十三五"厦门市海洋经济创新发展示范项目(16CZB020SF11)~~

摘　　要：目的建立QuEChERS EMR-Liqid-超高效液相色谱-串联质谱法测定动物源食品中的安眠酮残留的分析方法。方法样品用1%甲酸乙腈提取,经过QuEChERS EMR-Lipid萃取净化后,采用在电喷雾正离子模式下以质谱多反应监测(multiple reaction monitoring,MRM)方式检测。结果安眠酮在0.1~100 ng/m L浓度范围内线性良好,相关系数为0.9997,方法检出限0.03μg/kg,定量限为0.1μg/kg,在0.1、0.2和0.5μg/kg 3个加标水平下的回收率为85.5%~98.6%,相对标准偏差(relative standard deviations,RSDs)为2.07%~4.56%。结论该方法简便快捷、灵敏可靠,适用于动物源食品中的安眠酮药物的快速检测。Objective To establish a method for determination of methaqualone residues in animal-derived food by Qu ECh ERS EMR-Liqid-ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）.Methods Samples were extracted with acetonitrile with 1% formic acid,then purified by Qu ECh ERS EMR-Lipid extraction column.The UPLC-MS/MS（ESI＋） with multiple reactions monitoring（MRM） was used for detection.Results Methaqualone had a good linear relationship in the range of 0.1-100 ng/m L,and the correlation coefficient was 0.9997.The limit of detection was 0.03 μg/kg,and the limit of quantization was 0.1 μg/kg.The recoveries were ranged from 85.5%-98.6% for methaqualone at 3 spiked levels of 0.1,0.2 and 0.5 μg/kg.The relative standard deviations（RSDs） were from 2.07%-4.56%.Conclusion The method is easy,rapid,accurate and sensitive,which is suitable for detecting methaqualone in animal-derived food.

关 键 词：QUECHERS EMR-Liqid技术 安眠酮 超高效液相色谱-串联质谱法 动物源食品 

分 类 号：O657.63[理学—分析化学] TS207.5[理学—化学]