玉米赤霉烯酮降解菌ASAGW-10产芽孢条件研究  

Optimization of spore production condition of zearalenone-degrading strain ASAGW-10

作　　者：刘虎军[1] 常晓娇[1] 王楠希 赵一凡 杜稳 孙长坡[1] LIU Hu - jun;CHANG Xiao - jiao;WANG Nan - xi;ZHAO Yi - fan;DU Wen;SUN Chang - po(Academy of State Administration of Grain,Beijing 100037)

机构地区：[1]国家粮食局科学研究院,北京100037

出　　处：《粮油食品科技》2018年第4期50-54,共5页Science and Technology of Cereals,Oils and Foods

基　　金："十二五"国家科技支撑计划(2015BAK43B03)

摘　　要：通过培养基筛选、单因素实验、正交实验获得芽孢率和芽孢量较高的培养基,优化发酵pH和温度,获得最优产芽孢培养条件,在20 L发酵罐中对发酵培养基和发酵条件进行验证,并对发酵pH的控制和补料发酵进行初步探索。获得的最佳培养基为:麸皮5 g/L、玉米粉3 g/L、NaCl 5g/L、KH_2PO_41 g/L、MnSO_4·H_2O 0.2 g/L、K_2HPO_41.5 g/L,在pH 6.0、37℃、200 r/min条件下震荡培养21 h,芽孢率达到91%,芽孢量达到5.2×10^(10)CFU/mL。在20 L发酵罐放大实验中通过恒定pH和批次补料发酵,芽孢率达到95%,芽孢量达到1.38×10^(11)CFU/mL。获得了玉米赤霉烯酮降解菌ASAGW-10菌剂的生产工艺,为后续开展降解菌性能和应用研究提供数据支撑。The medium with high sporulation rate and quantity was obtained by selecting medium component,single factor experiment and orthogonal experiment,and the pH and temperature was optimized for the optimal culture condition. The medium and condition were verified in 20 L fermenter and the controlling of pH and fed-batch fermentation was explored. The optimal culture medium was： wheat bran5 g/L,corn flour 3 g/L,NaCl 5 g/L,KH2 PO41 g/L,MnSO4·H2 O 0. 2 g/L and K2 HPO41. 5 g/L.Cultured in shake flask with pH 6,200 r/min,at 37 ℃ for 21 h,the sporulation rate reached to 91%and sporulation quantity 5. 2 × 10^10 CFU/mL. In larger experiment,cultured in 20 L fermenter with constant pH and fed-batch fermentation,the sporulation rate reached to 95% and sporulation quantity 1. 38× 10^11 CFU/mL. The production process of the ZEN-degrading strain ASAGW-10 was obtained,which provided the guarantee for the subsequent study on the degrading bacteria and application.

关 键 词：玉米赤霉烯酮 枯草芽孢杆菌 芽孢 培养条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]