丹桂4个花期挥发性成分GC-MS分析及清除DPPH自由基活性的研究  

Analysis of Chemical Components by GC-MS and Scavenging Capacity against DPPH Free Radical of Volatile Oil from Four Flower Phases of Osmanthus Fragrans Var Aurantiacus

作　　者：司志国 丁志伟[1] 胡文杰[1] SI Zhi-guo;DING Zhi-wei;HU Wen-jie(College of Life Sciences,Jinggangshan University,Ji＇an 343009,China;Herman Vocational and Technical College,Environmental Art Engineering,Zhengzhou 450046,China)

机构地区：[1]井冈山大学生命科学学院,吉安343009 [2]河南职业技术学院环境艺术工程系,郑州450046

出　　处：《天然产物研究与开发》2018年第7期1111-1118,共8页Natural Product Research and Development

基　　金：江西省高等学校科学技术项目(GJJ170651);井冈山大学博士科研启动项目(JZB1309)

摘　　要：采用水蒸气蒸馏法提取丹桂花不同时期的挥发油,结合气相色谱-质谱联用技术对其进行分析和鉴定,用面积归一化法测定各组分的相对百分含量,并对该挥发油清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力进行了研究。结果表明:丹桂初花期、盛花初期、盛花期和末花期4个时期分别鉴定出29、30、34和27种化学成分,共鉴定出61种化合物,包括萜烯类15种、醇类15种、醛类6种、酮类9种、酯类8种、烷烃类6种、炔烃1种及氧化物类化合物1种,它们共有成分有8种,此外还检测到一些特有成分,初花期14种、盛花初期5种、盛花期5种和末花期4种。丹桂花挥发油具有一定清除DPPH自由基的能力,但其清除能力低于同质量浓度的Vc。The essential oils of Osmanthus fragrans var aurantiacus were obtained from different phases by steam distillation,which were analyzed by GC-MS,and the relative content of each component was determined by peak area normalization methods. The antioxidant activity of the essential oils was also investigated through the measurement of 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（ 2,4,6-trinitrophenyl） hydrazyl（ DPPH） scavenging activity. The results were as follows： there were 29,30,34 and 27 compounds,identified at start of flowering,start of peak season of flowering,peak season of flowering and end of flowering during different flower development stages in Osmanthus fragrans var aurantiacus,respectively. In total,61 components were identified from the volatile oils of four different development phases including terpenes（ 15）,alcohols（ 15）,aldehydes（ 6）,ketones（ 9）,esters（ 8）,alkanes（ 6）,alkyne（ 1） and oxides cervidaes（ 1）. Only eight common components were detected in four flower phases of Osmanthus fragrans var aurantiacus,in addition,the numbers of special components among four phases were 14,5,5 and 4 components,respectively.Volatile oil from Osmanthus fragrans var aurantiacus showed antioxidant activity,and the capacity of scavenging DPPH radicals of the volatile oils lower than that of Vc at the same mass concentration.

关 键 词：丹桂 挥发油 化学成分 气相色谱-质谱 抗氧化能力 

分 类 号：R931.6[医药卫生—生药学] Q946[医药卫生—药学]