CD68、S100、CD1α和Langerin在成人骨受累朗格汉斯细胞组织细胞增生症中的表达及临床意义  

Expressions and clinical significants of CD68,S100,CD1α and Langerin on adult bone involvement Langerhans cell histiocytosis

作　　者：王翠玲 李开智[2] 崔文 WANG Cui-ling;LI Kai-zhi;CUI Wen(Ji Nan University,Jinan Shandong 250012,China;Jining Medical College Affiliated Hospital,Jining Shandong 272029,China;Jining Medical College School of Forensic Medicine and Medical Laboratory,Jining Shandong 272029,China.)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院山东省医学科学院血液科实验室,山东济南250012 [2]济宁医学院附属医院病理科,山东济宁272029 [3]济宁医学院法医学与医学检验学院教研室,山东济宁272029

出　　处：《临床和实验医学杂志》2018年第15期1612-1617,共6页Journal of Clinical and Experimental Medicine

摘　　要：目的探究白细胞分化抗原68(CD68)、S100、白细胞分化抗原1α(CD1α)和Langerin在成人骨受累朗格汉斯细胞组织细胞增生症(LCH)中的表达,及其与患者临床特征、预后的关系。方法回顾性收集68例LCH患者组织标本进行研究,另选取32例其他非LCH组织细胞疾病(N-LCHD)患者标本作为对照组。采用Eli Vision TM Super/HRP法检测组织中CD68、S100、CD1α和Langerin的表达,分析上述四项指标与患者临床病理参数的关系,Kaplan-Meier法构建生存曲线分析CD68、S100、CD1α和Langerin与患者预后的关系。结果 CD68定位于细胞质,S100定位于细胞核/细胞质,CD1α和Langerin定位于细胞膜。与N-LCHD患者相比,CD68、S100、CD1α、Langerin阳性表达率均显著升高(P<0.05)。CD68灵敏度为92.65%,特异度为81.25%;S100灵敏度为97.06%,特异度为65.63%;CD1α灵敏度为94.12%,特异度为84.38%;Langerin灵敏度为95.59%,特异度为90.63%,四项指标灵敏度、特异度对比,差异无显著性(P>0.05)。CD68、S100、CD1α、Langerin表达与患者性别、骨病灶数量无关(P>0.05),与年龄、器官功能损害、骨受累分布相关(P<0.05)。68例患者随访36个月,共死亡46例,术后总体生存率为32.35%。Kaplan-Meier生存分析显示,CD68、S100、CD1α、Langerin阳性表达组生存率显著低于阴性组(χ~2=5.736,P=0.018;χ~2=4.808,P=0.036;χ~2=4.921,P=0.032;χ~2=5.304,P=0.027)。结论 CD68、S100、CD1α、Langerin在LCH患者中均呈阳性表达,与患者预后有关,对LCH患者临床治疗和预后判断有指导作用。Objective To investigate the expressions of cluster of differentiation 68（ CD68）,S100,cluster of differentiation 1α（ CD1α）,and Langerin on adult bone involvement Langerhans cell histiocytosis（ LCH）,and their relationships with clinical characteristics and prognosis of patients. Methods Tissue samples from 68 patients with LCH in our hospital were collected for study,and 32 patients with other non-LCH histiocytic cell disease（ N-LCHD） were selected as control groups,Eli Vision TM Super/HRP was used to detect the expressions of CD68,S100,CD1α,and Langerin in the tissues,analyzed the relationships between the above four indicators with clinical pathological parameters of patients,Kaplan-Meier method was used to construct the survival curve and analyzes the relationships between CD68,S100,CD1α and Langerin with prognosis. Results CD68 was located in the cytoplasm,S100 was located in the nucleus/cytoplasm,CD1α and Langerin were located in the cell membrane. Compared with N-LCHD patients,the positive expression rates of CD68,S100,CD1α and Langerin were significantly higher（ P〈0. 05）. The sensitivity of CD68 was 92. 65% and the specificity was 81. 25%; the sensitivity of S100 was 97. 06% and the specificity was 65. 63%; the sensitivity of CD1α was 94. 12% and the specificity was 84. 38%; the sensitivity of Langerin was 95. 59% and the specificity was 90. 63%. There was no significant difference in the sensitivity and specificity of the four indicators（ P〈0. 05）. The expressions of CD68,S100,CD1α,and Langerin were not related to gender and number of bone lesions（ P〈0. 05）,but were related to age,organ dysfunction,and distribution of bone involvement（ P〈0. 05）. Sixty-eight patients were followed up for 36 months,and a total of 46 deaths occurred,the postoperative overall survival rate was 32. 35%. Kaplan-Meier survival analysis showed that the survival rate of the CD68,S100,CD1α,Langerin positive expression group was significantly lower than that of the negative group（ χ

关 键 词：朗格汉斯细胞组织细胞增生症 白细胞分化抗原68 白细胞分化抗原1α S100蛋白 LANGERIN 

分 类 号：R730.43[医药卫生—肿瘤] R733.3[医药卫生—临床医学]