脉冲电场协同金属离子对卵清蛋白溶液电化学性质的影响  

Effects of pulsed electric field and metal ions on the electrochemical properties of ovalbumin solution

作　　者：胡菲 汤婷[1] 张佳 刘燕燕[1] 蒋爱民[1] 蒋卓[1] HU Fei;TANG Ting;ZHANG Jia;LIU Yah-yan;JIANG Ai-min;JIANG Zhuo(College of Food Science,South China Agricultrual University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品与机械》2018年第5期6-10,117,共6页Food and Machinery

基　　金：广东省自然科学基金(编号:2016A030310455)

摘　　要：采用zeta电位分析仪、电导率仪和电化学工作站,分析添加不同二价金属离子(Ca^(2+)、Ba^(2+)、Mn^(2+)和Cu^(2+))的卵清蛋白溶液在脉冲电场处理前后的zeta电位,电导率和氧化还原电位的变化情况,探究金属离子和PEF协同对蛋白质电化学性质的影响机制。结果表明:(1)添加了Ba^(2+)和Cu^(2+)的卵清蛋白溶液的zeta电位,随着脉冲电场能量的加入呈减小趋势,添加了Ca^(2+)和Mn^(2+)的样品呈先增大后减小的趋势;(2)随着脉冲电场处理时间增加,添加金属离子的蛋白溶液的电导率均呈先增大后减小的趋势,其中添加Ca^(2+)对蛋白溶液的电导率影响最大;(3)4种金属离子的添加都使脉冲电场处理后样品电活性下降,可导电物质的数量减少;随着处理次数增加,氧化还原电位都呈现不同程度的下降。Zeta potential analyzer,conductivity meter and electrochemical workstation were used to analyze the variation of zeta potential,electrical conductivity and redox potential,with addition of different divalent metal ions（Ca^2＋,Ba^2＋,Mn^2＋and Cu^2＋）to ovalbumin solution before and after pulsed electric field treatment.The results showed that：（1）the zeta potential of ovalbumin solution with Ba^2＋and Cu^2＋decreased with the addition of pulsed electric field energy and samples with Ca^2＋and Mn^2＋increased first and then decreased;（2） With the increase of pulse electric field treatment time,the conductivity of protein solution with metal ions increased first and then decreased,among which Ca^2＋had the greatest effect on the conductivity of protein solution;（3） the electrical activity and quantity of conductive materials decreased with the addition of four metal ions.Besides,the values of redox potential reduced gradually with increasing the treatment times.

关 键 词：脉冲电场 卵清蛋白 金属离子 ZETA电位 电导率 氧化还原电位 

分 类 号：TS201.21[轻工技术与工程—食品科学]