流动注射化学发光法在线检测饮用水中脱氧雪腐镰刀菌烯醇  

On-line determination of emergency pollution of deoxynivalenol in drinking water by flow injection chemiluminescence

作　　者：杨盼盼 唐书泽[1] 吴事正 李梁 滕久委 YANG Pan-pan;TANG Shu-ze;WU Shi zheng;LI Liang;TENG Jiu-wei(Department of Food Science and Engineering,Jinan University,Guangzhou,Guangdong 510632,China;International College,Jinan University,Guangzhou,Guangdong 510632,China)

机构地区：[1]暨南大学理工学院食品科学与工程系,广东广州510632 [2]暨南大学国际学院,广东广州510632

出　　处：《食品与机械》2018年第5期70-76,共7页Food and Machinery

基　　金：粤港联合创新项目(编号:2016A050503031)

摘　　要：为应对突发性脱氧雪腐镰刀菌烯醇污染饮用水事件的发生,基于脱氧雪腐镰刀菌烯醇对鲁米诺—过氧化氢体系的促进作用,建立流动注射化学发光在线检测脱氧雪腐镰刀菌烯醇的新方法。在优化的试验条件下,该方法测定脱氧雪腐镰刀菌烯醇的线性范围为0.001~4.000 mg/L;检出限(S/N=3)为0.001 mg/L;相对标准偏差(RSD,n=11)为1.89%;不同加标水平下的加标回收率为67.33%~94.50%;相对标准偏差(n=3)小于2.34%。该方法具有灵敏度高、分析速度快及操作简便等优点,可应用于突发性脱氧雪腐镰刀菌烯醇污染的在线快速检测和应急预警。A rapid,simple and sensitive flow injection chemiluminescence（CL）method was developed for the determination of deoxynivalenol in drink water.The method was based on the enhancement effect of deoxynivalenol on the CL reaction of luminol-H2 O2 in alkaline medium.The influences of various experimental factors,including sodium hydroxide,luminol,hydrogen peroxide,Tween 80 concentrations were investigated.Under the optimum conditions,the enhanced CL intensity was linearly related to the concentration of deoxynivalenol in the range of 0.001～4.000 mg/L with a detection limit（3σ）of 0.001 mg/L.The precision was calculated by analyzing sample containing 0.2 mg/L deoxynivalenol（n=11）and the relative standard deviation（RSD）was 1.89%.The recovery rates were67.33%～94.50% under different standard levels.This technique method could be applied to the on-line detection and emergency warning of sudden contamination of deoxynivalenol.

关 键 词：流动注射 化学发光 脱氧雪腐镰刀菌烯醇 在线检测 

分 类 号：O657.3[理学—分析化学] R123.1[理学—化学]