太空茄子叶黄酮大孔树脂纯化工艺优化及抗氧化性研究  

Purification of flavonoids from eggplant leaves using macroporous resin and its antioxidant activities

作　　者：杨彬[1] 金小青 李彩霞[1] 焦扬[1] YANG Bin;JIN Xiao-qing;LI Cai-xia;JIAO yang(College of Agriculture and Biotechnology Hexi University,Zhangye,Gansu 734000,China;Key Laboratory of Hexi Corridor Resourses Utilization of Gansu,Zhangye,Gansu 734000,China)

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖734000

出　　处：《食品与机械》2018年第5期158-163,共6页Food and Machinery

基　　金：国家大学生创新创业训练计划项目(编号:201510740001);甘肃省科技支撑计划-农业类(编号:144NKCA241)

摘　　要：通过静态吸附与解吸附试验确定纯化太空茄叶黄酮的大孔树脂类型并优化其工艺条件,对纯化前后太空茄子叶黄酮的抗氧化活性进行对比分析。结果表明,NKA-II为最佳树脂;NKA-II纯化太空茄子叶黄酮的适宜条件:粗黄酮上样浓度0.362 6mg/mL、上样液pH 2、静态吸附2.5h、用70%的乙醇(pH=3)解吸40 min,黄酮含量由纯化前17.91%上升到64.59%,纯化率提高3.61倍;与芦丁比较,纯化后黄酮对ABTS^+·、DPPH·的清除能力均小于芦丁,且纯化后黄酮对ABTS^+·的清除能力高于纯化前。Static adsorption and desorption experiments were carried out to determine the types of macroporous resins for purifying flavonoids from eggplant leaves, to optimize its technological conditions,and to comparatively analyze the antioxidant activities of eggplant leave flavonoids before and after purification.The results showed that NKA-Ⅱ was the optimal adsorbent.The optimum conditions for purification of flavonoids from eggplant leaves were listed as follows：sample concentration of crude extracts 0.362 6 mg/mL,sample pH 2,the static absorption time,2.5 h.After 40 minutes desorption with 70% ethanol（pH=3）,the content of flavonoids was increased from 17.91% before purification to 64.59%,the rate of purification was improved by 3.61 times.Compared with rutin,scavenging activity of flavonoids after purification on ABTS^＋· and DPPH· was less,and scavenging activity of flavonoids after purification on ABTS^＋· was stronger than before purification.

关 键 词：茄子叶 大孔树脂 黄酮 纯化 抗氧化 

分 类 号：TQ28[化学工程—有机化工]