α-L-鼠李糖苷酶高密度发酵及其固定化  

High density fermentation of α-L-rhamnosidase and enzyme immobilization

作　　者：刘嘉男 巩建业 高庭 李利君 倪辉 LIU Jia-nan, GONG Jian-ye, GAO Ting, LI Li-jun , NI Hui(College of Food and Biological Engineering, Jimei University, Xiamen 361021, Chin)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021

出　　处：《食品与发酵工业》2018年第7期44-48,54,共6页Food and Fermentation Industries

基　　金：国家自然基金(31371751)

摘　　要：在5 L发酵罐中对α-L-鼠李糖苷酶进行小试发酵,制备酶液;以聚乙烯醇-海藻酸钠为包埋载体,以戊二醛为交联剂对该酶进行固定化。经5 L罐高密度发酵96 h,α-L-鼠李糖苷酶酶活为2 766 U/g,生物量为228g/L。经过固定化后,α-L-鼠李糖苷酶的酶活为20 U/g,酶活回收率为32.88%,固定化α-L-鼠李糖苷酶的最适反应温度为35~50℃,最适反应p H值为4.0,该固定化酶在连续使用6次后酶活仍然保留98.22%。高密度发酵能显著提高酶的产量,同时固定化技术提高了酶的重复利用率,为该酶利用芦丁酶法转化制备异槲皮苷提供了依据。α-L-rhamnosidase produced by high density fermentation was immobilized into matrix consisting of polyvinyl alcohol and sodium alginate with glutaraldehyde as crosslinker. In the 5 L bioreactor,the activity of immobilized enzyme achieved 2 766 U/g and its biomass reached 228 g/L after 96 h cultivation. The enzyme activity recovery of 32. 88% was obtained and the activity of immobilized enzyme was 20 U/g. The optimal temperature was 35-50 ℃ and the optimal reaction p H at about 4. 0 for immobilized enzyme. The residual enzyme activity could retain more than 98. 22% after 6 batches of recycling use. These results suggested that the high density fermentation could effectively enhance enzyme production,and the immobilization technology could improve enzyme recycling rate,which would offer a basis for large-scale production of isoquercitrin.

关 键 词：α-L-鼠李糖苷酶 高密度发酵 酶固定化 异槲皮苷 

分 类 号：TQ925[轻工技术与工程—发酵工程]