青海地区结核分枝杆菌链霉素耐药基因rpsL和gidB突变特征  

Mutation characteristic of the rpsL and gidB genes resistant to streptomycin in isolates of Mycobacterium tuberculosis from Qinghai area

作　　者：申秀丽[1] 王兆芬[1] 李斌[1] 蒋明霞[2] 张媛媛 李会茹 郭术勤 康楚婕 SHEN Xiu-li;WANG Zhao-fen;LI Bin;JIANG Ming-xia;ZHANG Yuan-yuan;LI Hui-ru;GUO Shu-qin;KANG Chu-jie(Department of Public Health,Medical School,Qinghai University,Xining 810008,China;Institute for Communicable Disease Control and Prevention,Qinghai Provincial Center for Disease Control and Prevention,Xining 810007,Chin)

机构地区：[1]青海大学医学院公共卫生系,西宁810001 [2]青海省疾病预防控制中心传染病预防控制所,西宁810007

出　　处：《国际药学研究杂志》2018年第3期205-210,共6页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81660556)

摘　　要：目的探讨青海地区链霉素耐药结核分枝杆菌基因突变特征。方法收集青海省192株结核分枝杆菌,采用比例法检测其表型耐药情况,PCR扩增链霉素耐药相关基因,进行基因测序并分析结果。结果链霉素耐药结核分枝杆菌rps L基因的rps LK43R(AAG→AGG)和rps LK88R(AAG→AGG)位点突变率高于敏感菌株(χ~2=70.58,P<0.001),rps LD14E(GAC→GAG)位点突变主要发生在链霉素敏感的菌株中;rps L基因突变检测链霉素耐药的敏感度为56.41%,特异度为95.61%;结核分枝杆菌gid BE92D和gid BA205A位点突变与结核分枝杆菌耐链霉素没有关系(χ~2=0.40,P=0.528),北京基因型结核分枝杆菌gidBE92D和gid BA205A位点突变率高(χ~2=123.33,P<0.001)。结论基因测序rps L基因可快速诊断结核分枝杆菌链霉素耐药,gid B基因E92D和(或)A205A位点检测可用于结核分枝杆菌北京/非北京基因型的鉴定。Objective To explore the characteristics of gene mutation of streptomycin-resistant Mycobacterium tuberculosis in Qinghai area. Methods Totally 192 strains of M. tuberculosis were collected,and drug-resistant phenotypes were detected by the proportion method. Genes related to the streptomycin-resistance were amplified by the PCR method,and then sequenced and analyzed.Results The mutation rates of rps LK43 R（AAG→AGG）and rps LK88 R（AAG→AGG）in streptomycin-resistant M. tuberculosis were higher than those in sensitive strains（χ^2=70.58,P〈0.001）. The rps LD14 E（GAC→GAG）mutation mainly occurred in streptomycin-sensitive strains,the sensitivity of rps L gene mutation for detecting streptomycin resistance was 56.41% and the specificity was95.61%. Mutations in gid BE92 D and gid BA205 A site of M. tuberculosis were not correlated to the streptomycin-resistance in Mycobacterium tuberculosis（χ^2=0.40,P=0.528）. The mutation rates of gid BE92 D and gid BA205 A in Beijing genotype M. tuberculosis were higher than those in non-Beijing genotype M. tuberculosis（χ^2=123.33,P〈0.001）. Conclusion The rps L gene sequencing may be used for rapid clinical diagnosis of the streptomycin-resistance of M. tuberculosis. The detection of gid BE92 D and/or gid BA205 A mutations could be used for identification of the Beijing and non-Beijing genotypes M. tuberculosis.

关 键 词：结核分枝杆菌 链霉素耐药 基因测序 基因突变 

分 类 号：R52[医药卫生—内科学]