大豆渣制备细菌纤维素的研究  

Preparation of Bacterial Cellulose with Soybean Residues

作　　者：高媛[1,2] 邹小周 洪枫 陈琳[2] GAO Yuan;ZOU Xiao-zhou;HONG Feng;CHEN Lin(State Key Laboratory for Modification of Chemical Fibers and Polymer Materials（Donghua University）,Shanghai 201620,China;College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]纤维材料改性国家重点实验室(东华大学),上海201620 [2]东华大学化学化工与生物工程学院,上海201620

出　　处：《纤维素科学与技术》2018年第2期17-23,30,共8页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金(51373031);上海市科委项目(15520720800);纤维材料改性国家重点实验室资助课题;中央高校基本科研业务费专项资金资助项目

摘　　要：细菌纤维素(BC)是一类由微生物产生的纳米纤维素,其偏高的生产成本,尤其是成本相对较高的发酵碳源,限制了其工业化生产和应用。以廉价丰富的大豆渣为原料,进行稀酸预处理,剩余的不溶性滤渣经纤维素酶的水解获得可发酵糖。分别以酸预处理液和酶水解液配制培养基,用来制备细菌纤维素。结果表明:以酶解液作为发酵碳源的BC产量明显高于以酸预处理液为碳源时的产量;且以Ca(OH)2处理的酶解液为碳源时,BC产量可达5.52 g/L,远高于葡萄糖对照组(7.4倍),表明大豆渣作为碳源生产BC具有良好的应用前景和商业价值。Bacterial cellulose（BC） is nano cellulose produced by microorganisms. Its high production cost, especially the cost of carbon source needs to be decreased for its industrialization and commercial application. Through use of cheap and rich soybean residues as raw materials, which are waste after extraction of oil and protein, fermentable sugars were obtained by enzymatic hydrolysis of the cellulose residues after dilute acid pretreatment of the soybean residues. The acid pretreatment liquid and enzymatic hydrolysate was tried to be used as carbon source for production of bacterial cellulose, respectively. The results showed that the yield of BC with enzymatic hydrolysate as the carbon source was significantly higher than that from acid pretreatment liquid. BC production with Ca（OH）2-treated enzymatic hydrolysate reached up to 5.52 g/L, which was 7.4 times higher than that from a control of glucose-based media.

关 键 词：细菌纤维素 大豆渣 酸预处理 酶水解 可发酵糖 

分 类 号：Q81[生物学—生物工程]