苹果中福美胂残留的RP-HPLC检测  

Determination of residual asomate in apple by reversed-phase high-performance liquid chromatography(RP-HPLC)

作　　者：牛鹏飞[1] 申远[1] 李帅[1] 郭玉蓉[1] NIU Peng-fei;SHEN Yuan;LI Shuai;GUO Yu-rong(College of Food Engineering and Nutritional Science,Shar~i Normal University,Xi＇ an 710062,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062

出　　处：《江苏农业学报》2018年第3期706-710,共5页Jiangsu Journal of Agricultural Sciences

基　　金：农业部苹果产业技术体系项目(NYCYTX-08)

摘　　要：为定量检测苹果中福美胂残留,建立一种基于紫外检测器的反相高效液相色谱检测方法。以苯为溶剂,震荡萃取苹果中福美胂,采用C_(18)色谱柱(250.0 mm×4.6 mm ID,5.0μm),以0.10%三氟乙酸水溶液为溶剂配制乙腈含量为10%溶液作为A相,100.00%乙腈为B相(A相∶B相=60∶40,体积比),在流速1.00 ml/min,柱温30℃,波长247 nm条件下进行福美胂色谱分析。结果表明,福美胂的加标回收率为76.56%~100.22%,变异系数(CV)为4.86%~7.51%,检出限(LOD)为0.295 3 mg/kg,定量限(LOQ)为1.206 7 mg/kg。该方法可用于苹果中福美胂残留检测。A reversed-phase high-performance liquid chromatography（ RP-HPLC） separation method with ultraviolet detector was developed to determine the residual asomate in apple. Using benzene as solvent,the asomate in apple was extracted. The asomate was determined by RP-HPLC using C（18）（ 250.0 mm×4.6 mm ID,5.0 μm） column and mobile phase consisting of A and B in the ratio of 60 ∶ 40（ V/V） with a flow rate of 1.00 ml/min at 30 ℃ in 247 nm. A was 0. 10% trifluoroacetic acid aqueous solution-acetonitrile,B was 100. 00% acetonitrile. The results showed that spiked recovery rate of asomate ranged from 76.56% to 100.22%,and coefficient of variation（ CV） ranged from 4.86% to 7.51%. The limit of detection（ LOD） was 0.295 3 mg/kg,and the limit of quantification（ LOQ） was 1.206 7 mg/kg in apple. This method can be successfully used to measure residual asomate in apple.

关 键 词：RP-HPLC检测 福美胂 苹果 

分 类 号：X836[环境科学与工程—环境工程]