莲房原花青素改善老化大鼠学习记忆功能的机制  

Procyanidins extracted from the lotus seedpod improve the learning and memory abilitiesin cognitively impaired aged rats

作　　者：聂淑科 高慧 许继取[3] 张振涛[1] 张允建 刘烈刚[5] 张兆辉[1] NIE Shutee;GAO Hui;XU Jiqu;ZHANG Zhentao;ZHANG Yunjian;LI Liegang;ZHANG Zhaohui(Department of Neurology,Renrnin Hospital of Wuhan University,Wuhan Hubei 430066,China)

机构地区：[1]武汉大学人民医院神经内科,430060 [2]华中科技大学同济医学院附属同济医院营养科,430030 [3]中国农业科学院油料作物研究所,430062 [4]华中科技大学同济医学院附属协和医院神经内科,430022 [5]华中科技大学同济医学院公共卫生学院营养与食品卫生系,430030

出　　处：《中国神经免疫学和神经病学杂志》2018年第4期279-283,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金青年项目(No.81701257);武汉大学自主科研项目(2042017kf0050)

摘　　要：目的观察莲房原花青素(LSPC)对老年脑老化大鼠学习记忆能力的影响,并探讨血红素氧合酶(HO)系统在其中的可能作用机制。方法以40只4月龄年轻雌性SD大鼠Morris水迷宫实验第3~5天的平均潜伏期为基础,从100只18月龄雌性SD大鼠中筛选出老年认知功能正常(aged unimpaired,AU)大鼠(其平均潜伏期小于年轻大鼠平均潜伏期3个标准差)和老年认知障碍(aged impaired,AI)大鼠(其平均潜伏期大于年轻大鼠0.5个标准差)。随机选取12只4月龄大鼠为年轻对照组,随机选取12只AU大鼠为AU组,随机选取36只AI大鼠并随机分为AI组、低剂量LSPC(L-LSPC,50mg/kg)干预组、高剂量LSPC(H-LSPC,100mg/kg)干预组。采用Morris水迷宫试验评估各组大鼠的学习记忆能力,采用ELISA法检测各组大鼠皮质、海马中HO-1及HO-2表达水平。结果 AI组大鼠Morris水迷宫实验平均潜伏期较年轻对照组大鼠明显延长(P<0.05),L-LSPC和H-LSPC干预组大鼠第3~5天平均潜伏期和游泳距离均明显短于AI组(P<0.05或P<0.01)。各组大鼠皮质HO-1和HO-2表达水平组间比较差异无统计学意义(均P>0.05)。各组大鼠海马组织HO-1和HO-2表达存在统计学差异(均P<0.01),与年轻对照组和AU组比较,AI组大鼠海马HO-1水平明显升高(均P<0.01),而海马HO-2水平降低(均P<0.05);与AI组比较,L-HSPC和H-LSPC组海马HO-1水平降低(均P<0.01),而海马HO-2表达水平升高(P<0.01),且H-LSPC组升高海马HO-2水平较L-LSPC组更明显(P<0.01)。结论 LSPC可明显改善老年脑老化大鼠的记忆功能,其机制可能通过降低大脑海马组织HO-1水平、上调HO-2表达水平来发挥神经保护作用。Objective The major purpose of this study was to investigate the effect of lotus seedpod procyanidins（LSPC）on the heme oxygenase（HO）systemin cognitively impaired aged rats.Methods Based on the average latency of 40 four-month-old young female SD rats in the Morris water maze（MWM）experiment on the 3-5 th day,rats in aged unimpaired（AU）group（mean latency score was0.5 SD from the mean of young controls）and aged impaired（AI）group（mean latency score was3.0 SDs from the mean of young controls）were randomly selected from 100 18-month-old female SD rats.The rats in the young control group（n=12）and AU group（n=12）were randomly selected from the young rats and AU rats,respectively.The rats categorized as AI were randomly subdivided into 3 groups consisting of 12 animals each：AI group,low-dose LSPC group（LLSPC,50 mg/kg）and high-dose LSPC group（H-LSPC,100 mg/kg）.The MWM was performed to assess the learning and memory abilities of the rats in all experimental groups.The levels of HO-1 and HO-2 in the cortex and hippocampus of rats in each group were evaluated by ELISA kits.Results Rats in the AI group exhibited a longer escape latency than that in the young group during all training days（P〈0.05）.Rats in the LLSPC and the H-LSPC groups had significantly shorter escape latency and swimming distance compared with the AI group on days 3 to 5（P〈0.05 or P〈0.01）.No significant differences were found on the expression levels of HO-1 and HO-2 in the cortex among all the groups（P〈0.05）.There were significant differences among all five groups in the hippocampal expressions of HO-1 and HO-2（P〈0.01）.The levels of HO-1 in hippocampus of AI group were significantly higher compared with the young group and AU group（all P〈0.01）,and the level of HO-2 in hippocampus was lower than that of other groups（P〈0.05）.There was a substantial decrease in the hippocampal HO-1 level and a significant increase in the hippocampal HO-2 level in the L-HSPC group and HLSPC group

关 键 词：记忆 原花青素类 认知障碍 血红素氧化酶(脱环) 水迷宫实验 

分 类 号：R749.5[医药卫生—神经病学与精神病学]