山慈菇多糖的免疫调节作用及对小鼠骨肉瘤细胞S180体内生长抑制作用  

Effects of Pseudobulbus cremastrae seu pleiones Polysaccharide on the Regulation of Immune Function and the Inhibition of Tumor Growth in Sarcoma S180 Tumor-Bearing Mice

作　　者：姜爽[1] 徐婧瑶 苏鑫[1] 刘智[1] 宋伍 JIANG Shuang;XU Jingyao;SU Kin;LIU Zhi;SONG Wu(College of Basic Medical Sciences,Changchun University of Chinese Medicine,Changchun 130117,China;Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学基础医学院,吉林长春130117 [2]长春中医药大学附属医院,吉林长春130021

出　　处：《食品科学》2018年第13期216-221,共6页Food Science

基　　金：吉林省科技发展计划项目(20140520016JH);吉林省教育厅"十三五"科学技术研究项目(吉教科合字2016第27号)

摘　　要：目的:探讨山慈菇多糖的免疫调节作用及对小鼠骨肉瘤细胞S180的抑制作用。方法:噻唑蓝法及吞噬中性红法测定小鼠脾淋巴细胞增殖能力及脾巨噬细胞活性;体内肿瘤抑制实验检测山慈菇多糖对S180荷瘤小鼠肿瘤生长、脾脏指数和胸腺指数的影响;流式细胞术及酶联免疫吸附测定法检测山慈菇多糖对S180荷瘤小鼠T淋巴细胞亚群及血清细胞因子水平的影响。结果:山慈菇多糖可以增强淋巴细胞增殖能力和巨噬细胞活性;同时,山慈菇多糖也可以抑制S180荷瘤小鼠肿瘤生长,增加小鼠脾脏指数和胸腺指数;此外,山慈菇多糖还可以增加荷瘤小鼠CD4+、CD8+T细胞浓度,提高CD4+/CD8+比值及白细胞介素-2、肿瘤坏死因子-α、干扰素-γ水平。结论:山慈菇多糖具有明显的肿瘤抑制作用,其对肿瘤细胞生长的抑制作用与提高免疫能力有关。Objective： To explore the effects of Pseudobulbus cremastrae seu pleiones polysaccharide（PCSPP） on the regulation of immune functionand the inhibition of tumor growth in sarcoma S180 tumor-bearing mice. Methods： Lymphocyte proliferation capacity and the phagocytic activity of spleen macrophages were determined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide and neutral red methods; the effects of PCSPP on tumor growth, spleen index and thymus index in S180 tumor-bearing mice were evaluated through tumor suppression test in vivo, and the effects on T lymphocyte subgroup and serum cytokine levels were detected by flow cytometry and enzymelinked immunosorbent assay, respectively. Results： PCSPP could enhance lymphocyte proliferation capacity and macrophage phagocytic activity. PCSPP could inhibit tumor growth and increase spleen index and thymus index in S180 tumor-bearing mice; meanwhile, it could also increase the levels of CD4＋ and CD8＋ T cells in mice and elevate the CD4＋/CD8＋ ratio, interleukin-2, tumor necrosis factor-α and interferon-γ levels. Conclusion： PCSPP has a potent antitumor effect and its inhibitory effect may be related to ithe improvement of immune function.

关 键 词：山慈菇多糖 骨肉瘤 免疫调节 细胞因子 

分 类 号：R151.3[医药卫生—营养与食品卫生学]