左旋多巴的合成研究新进展  

Advanced Research Progress in L-DOPA Synthesis

作　　者：陈五九[1] 曹鹏 宋国田 彭彦峰[1] 吴凤礼 王钦宏[1] CHEN Wujiu;CAO Peng;SONG Guotian;PENG Yanfeng;WU Fengli;WANG Qinhong(CAS Key Laboratory of Systems Microbial Biotechnology,Tianjin Institute of Industrial Bio technology,Chinaese Academy of Sciences,Tianjin,300308,China)

机构地区：[1]中国科学院天津工业生物技术研究所中国科学院系统微生物工程重点实验室,天津300308

出　　处：《广西科学》2018年第3期274-278,共5页Guangxi Sciences

基　　金：国家重点基础研究发展计划(973计划)项目(2011CBA00800);天津市科技计划项目(14ZCZDSY00066)资助

摘　　要：左旋多巴(L-DOPA)是治疗帕金森病的最有效药物,在各国的临床中应用广泛。左旋多巴的制备方法包括化学合成、植物提取、酶催化以及微生物合成。化学合成法存在低转化率和环境污染问题。植物提取法不仅产量低,还受到资源限制。酶催化法使用包括酪氨酸酚解酶、酪氨酸酶及羟基苯乙酸3-羟化酶等催化苯类前体合成左旋多巴。随着合成生物学的发展,构建代谢工程微生物、发酵法生产左旋多巴是一种高效、环境友好的新方法,具有良好的应用前景。L-DOPA is the most effective drug for Parkinson＇s disease and has been widely used in clinical treatment of various countries. The preparation methods of L-DOPA include chemical synthesis, plant extraction, enzyme catalysis, and microbial synthesis. However, Chemical syn thesis has low conversion rate and environmental pollution problems. The plant extraction method not only has a low yield but is also subject to resource constraints. Enzymatic to L-DOPA has been catalyzed by tyrosine phenol lyase, tyrosinase and p-hydroxyphenylacetate 3 hydroxylase. The enzyme catalyzed method uses catalyzed benzene precursors such as tyrosine phenolase,tyrosinase and p hydroxyphenylacetate 3 hydroxylase to synthesize L-DOPA. With the development of synthetic biology,fermentation of L-DOPA by constructing metabolically engineered microorganisms would be a new high efficient and environment friendly method, which demonstrates a promising process.

关 键 词：左旋多巴 不对称合成 植物提取 酶催化 代谢工程 

分 类 号：Q939.97[生物学—微生物学] TQ92[轻工技术与工程—发酵工程]