RGS1在人黑色素瘤细胞中对PD-L1表达调控的相关性研究  

Study on the correlation between RGS1 and PD-L1 expression regulation in human melanoma cells

作　　者：王欣玮 孙梦妍 王宇翀 戴海英 徐建国 武铠 郭蕊 吴敏靓 薛春雨 WANG Xin-wei;SUN Meng-yan;WANG Yu-chong;DAI Hai-ying;XU Jian-guo;WU Kai;GUO Rui;WU Min-liang;XUE Chun-yu(Department of Plastic Surgery,Changhai Hospital Affiliated to Naval Military Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学附属长海医院整形外科,上海200433

出　　处：《中国美容整形外科杂志》2018年第8期485-488,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：上海市科学技术委员会基础研究基金(13JC1401403)

摘　　要：目的研究RGS1在人黑色素瘤细胞中对PD-L1表达的调控和影响,进而探究RGS1对恶性黑色素瘤发生、发展的作用机制。方法⑴选取34组黑色素瘤临床样本,对每组标本的病理切片分别行抗RGS1和抗PD-L1免疫组化染色,并且分析在黑色素瘤细胞中,两种分子的表达是否具有相关性。⑵选取A375(人体黑色素瘤)细胞,构建RGS1过表达组、RGS1敲低组和对照组,分别测定各组细胞中PD-L1的转录和表达水平,并且分析RGS1对PD-L1是否具有调控作用。结果分别以色素痣的RGS1和PD-L1的免疫组化染色深度作为基准,将34例黑色素瘤临床样本染色分组。发现PD-L1与RGS1在人黑色素瘤细胞中的表达具有正相关性(P<0.05)。在细胞实验中,RGS1敲低组的PD-L1蛋白表达量出现明显降低;而RGS1过表达组的PD-L1蛋白表达量及mR NA表达量较对照组均出现明显增高。说明PD-L1的表达会随RGS1的表达变化而产生变化。结论在恶性黑色素瘤中,RGS1对PD-L1的表达可能具有正向调控作用。Objective To study the regulation and influence of RGS1 on PD-L1 expression in human melanoma cells, and to explore the mechanism of RGS1 on the occurrence and development of malignant melanoma. Methods（1） Thirty-four groups of melanoma samples were selected and subsequently underwent anti-RGS1 and PD-L1 immunohistochemical staining. The correlation between the expression of two molecules in melanoma cells was analyzed.（2） A375 cells（human melanoma cells） were selected to construct three groups： an RGS1 overexpression, knockdown, and control group. The transcription and expression level of PD-L1 in each group of cells was determined to analyze whether RGS1 has a regulatory effect on PD-L1. Results Based on the immunohistochemical staining depth of RGS1 and PD-L1 in pigmented nevus, 34 melanoma samples were stained and divided into 3 groups. The expression of PD-L1 and RGS1 in human melanoma cells was found to be positively correlated（P〈0.05）. In the cell experiment, the expression of PD-L1 protein in the RGS1 knockdown group decreased significantly, but the expression of PD-L1 protein and mR NA in the RGS1 overexpression group was significantly higher than that in the control group. It showed that the expression of PD-L1 will change with the change of RGS1 expression.Conclusion In malignant melanoma, the expression of PD-L1 was maybe positively regulated by RGS1.

关 键 词：黑色素瘤 RGS1 PD-L1 PD1 表达调控 

分 类 号：R739.5[医药卫生—肿瘤]