栀子油对小鼠急性酒精性肝损伤的保护作用  

Protective effect of Gardenia oil against alcohol-induced acute hepatic injury in mice

作　　者：漆乐媛 张风波[2] 肖日传 朱梓豪[2] 张俊逸 罗光明[2] QI Leyuan;ZHANG Fengbo;XIAO Richuan;ZHU Zihao;ZHANG Junyi;LUO Guangming(Nanchang County Hospital of TCM,Nanchang 330004,China;School of Pharmacy,Jiangxi University of TCM,Nanehang 330004,China)

机构地区：[1]南昌县中医院,南昌330004 [2]江西中医药大学药学院,南昌330004

出　　处：《中国油脂》2018年第8期76-80,共5页China Oils and Fats

基　　金：国家公益性科研专项(201507002)

摘　　要：研究栀子油对酒精所致小鼠急性肝损伤的保护作用。将72只小鼠随机分为正常组,模型组,栀子油低、中、高剂量组(0.3、0.6、1.2 g/kg)和阳性对照组(联苯双酯滴丸,200 mg/kg),每组12只,正常组和模型组灌胃等量的0.5%吐温-80溶液。采用改良后的造模方法(连续给药10 d后灌胃56%乙醇,15 m L/kg,连续灌胃2次)建立小鼠急性肝损伤模型,测定小鼠血清中AST、ALT、γ-GT和TG水平,检测肝组织匀浆中ADH、SOD、MDA、GSH和GSH-Px的含量,RT-PCR检测肝组织中TNF-αmRNA的表达量,HE染色光学显微镜分析小鼠肝损伤程度。结果表明:与模型组相比,栀子油各剂量组均能降低血清ALT、AST、γ-GT和TG的含量,提高ADH、GSH、GSH-Px、SOD活性,降低肝组织MDA的产生,下调TNF-αmRNA的相对表达量,肝组织变性及坏死等病理症状明显改善,尤以栀子油高剂量组效果最佳。栀子油对酒精所致小鼠急性肝损伤有保护作用,其保护机制可能与增强肝组织脂质代谢水平、提高抗氧化酶体系活力、抑制氧化应激反应及炎性因子表达相关。The protective effect of Gardenia oil against alcohol - induced acute hepatic injury in mice was investigated. Totally 72 mice were randomly divided into normal group, model group, low, medium and high dose group（0.3,0.6,1.2 g/kg） and positive control group（ bifendate pill, 200 mg/kg）, 12 mice in each group, and the normal group and the model group were treated with an equal amount of 0.5% Tween -80 solution. The acute liver injury model was induced by modified method, and the model, posi- tive control and experimental groups were orally administrated 56% alcohol（ 15 mL/kg） twice at 12 h in- terval on the tenth day after drugs administration. After 12 h, the mice were sacrificed to contribute blood and liver for biochemical and histological examinations. The results showed that compared with the model group, serum ALT, AST, T - GT and TG contents reduced, ADH, GSH, GSH - Px and SOD activitiesimproved, MDA generation and the relative expression of TNF -α mRNA decreased, and liver tissue degeneration and necrosis and other pathological symptoms improved significantly in each Gardenia oil dose group especially in the high dose group. The Gardenia oil had the protec-tive effect against alcohol - induced acute hepatic injury which might be depended on the enhancement of lipid metabolism level of liver tissue, improvement of the antioxidant enzyme system activity, inhibition of oxidative stress and reduction of inflammation factors expression.

关 键 词：栀子油 酒精性肝损伤 保肝作用 抗氧化 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] R965.1[轻工技术与工程—食品科学与工程]