香水莲花提取物的美白功效研究  

Study on Whitening Effect of Nymphaea hybrid Extracts

作　　者：孙玉洁[1] 李春松 芦芳 吴晓琴[1] 沈建福[1] SUN Yujie, LI Chunsong, LU Fang, WU Xiaoqin, SHEN Jianfu(1. College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058 . China ; 2. Department of Food and Drug Inspection and Evaluation ,Jinhua Institute for Food and Drug Contral, Jinhua 321015, China ; 3. Zhejiang Yunqilongxiang Biological Technology Co., Ltd., Hangzhou 310000, China)

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]金华市食品药品检验检测研究院食品药品检验评审科,浙江金华321015 [3]浙江芸麒龙翔生物技术有限公司,浙江杭州310000

出　　处：《食品与生物技术学报》2018年第7期776-783,共8页Journal of Food Science and Biotechnology

摘　　要：天然提取物由于其安全性高,近年来成为化妆品功效研究的热点。通过体外法测定香水莲花提取物所含活性成分及其对酪氨酸酶活性的抑制效果,再采用细胞模型探究其对B16小鼠黑素瘤细胞形态、增殖、黑素含量以及细胞内酪氨酸酶活性的影响。结果显示,黄色、蓝色香莲整花提取物均能有效抑制体外酪氨酸酶活性,其中体积分数60%醇提物能有效减缓B16黑素瘤细胞增殖、抑制黑素合成及胞内酪氨酸酶活性,添加100μg/m L的黄、蓝色整花体积分数60%醇提物的细胞增殖率分别为(84.44±1.77)%、(48.24±3.14)%,黑素质量分数为(69.69±1.40)%、(66.98±2.44)%,具有较好的美白功效。Due to the high safety of natural extracts, it becomes hotter on cosmetic efficacy study in recent years. The active ingredient and inhibition of tyrosinase activity in vitro of Nymphaea hybrid extracts are determined,then using the cell model of B16 mouse melanoma cell to observe cell morphology and assay proliferation rate, melanin content and intracellular tyrosinase activity of the yellow and blue whole flower extracts. The results show, the extracts can inhibit tyrosinase activity in vitro. 60% ethanol extracts of yellow flower and blue flower can effectively reduce cell proliferation rate and inhibit melanin synthesis as well as intracellular tyrosinase activity. The proliferation rate for high dose group （100 μg/mL） of the two extracts are respectively 84.44%±1.77%,48.24%±3.14% and melanin content are 69.69%±1.40%,66.98%±2.44%. So it is confirmed that Nymphae, hybrid Extracts have protective whitening effect.

关 键 词：香水莲花 提取物 美白 黑素瘤细胞 

分 类 号：TS974.1[轻工技术与工程]