响应面法优化复合酶提取灵芝总三萜工艺  

Optimization of Complex Enzyme Extraction Technology of Total Triterpenoids from Ganoderma lucidum by Response Surface Methodology

作　　者：丁霄霄 李凤伟 余晓红 DING Xiaoxiao;LI Fengwei;YU Xiaohong(School of Marine and Bioengineering,Yancheng Institute of Technology（Yancheng 224051;School of Food and Biological Engineering,J iangsu University（Zhenjiang 212013)

机构地区：[1]盐城工学院海洋与生物工程学院,盐城224051 [2]江苏大学食品与生物工程学院,镇江212013

出　　处：《食品工业》2018年第8期40-44,共5页The Food Industry

基　　金：江苏省科技厅苏北科技发展计划项目(富民强县)(BN2015123);江苏省产学研前瞻型联合研究项目(BY2016065-17;BY2016065-14)

摘　　要：以灵芝子实体为原料,研究复合酶(纤维素酶、半纤维素酶、木瓜蛋白酶)提取灵芝总三萜的工艺条件。在酶解时间、酶解温度和酶解pH三个单因素试验的基础上,利用响应面法优化复合酶提取灵芝总三萜的工艺条件。酶解提取工艺条件为:酶解处理时间、温度和pH分别为90 min、50℃和5,酶解处理后的原料继续用无水乙醇80℃水浴回流提取2 h。在此条件下灵芝总三萜的提取得率为1.29%±0.04%,与理论预测值1.28%接近。复合酶提取方法简单、效率高,可以用于提取灵芝总三萜。Ganoderma lucidum fruit body was used as raw materials to study complex enzyme（cellulose, hemicellulase and papain） extraction technology of total triterpenoids from Ganoderma lucidum. Response surface methodology was used to optimize the extraction process of complex enzyme of total triterpenoids from Ganoderma lucidum based on the results of three single factors of hydrodysis time, hydrodysis temperature and pH. The optimum extraction conditions were as follows： hydrodysis time 90 min, hydrodysis temperature 50℃ and pH 5. Then the enzyme hydrodysis treatmented materials was refluxed with ethanol for 2 h in 80℃. Under these conditions the yield of total triterpenoids from Ganoderma lucidum was 1.29%±0.04% closing to predicted extraction rate 1.28%. This extraction technique was simple, efficient and applicable for the extraction of total triterpenoids from Ganoderma lucidum.

关 键 词：响应面法 复合酶 提取 灵芝 三萜 

分 类 号：TQ28[化学工程—有机化工]