硫氰亚铁铵比色法测定蛋黄卵磷脂中总磷脂含量  

Determination of Total Phospholipids in Egg Yolk Lecithin by Thiocyanuric Ammonium Spectrophotometry

作　　者：龚金炎[1] 靳羽晓 王静静 李归浦 赵广生 肖功年[1] GONG Jinyan;JIN Yuxiao;WANG Jingjing;LI Guipu;ZHAO Guangsheng;XIAO Gongnian(Zhejiang Provincial Key Lab for Chem ＆ Bio Processing Technology of Farm Produces,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology（Hangzhou 310023;Beingrnate Baby ＆ Child Food Co.,Ltd（Hangzhou 311106;Hangzhou New Hope Bimodal Dairy Co.,Ltd（Hangzhou 311100)

机构地区：[1]浙江省农产品化学与生物加工技术重点实验室浙江科技学院生物与化学工程学院,杭州310023 [2]贝因美婴童食品股份有限公司,杭州311106 [3]杭州新希望双峰乳业有限公司,杭州311100

出　　处：《食品工业》2018年第8期319-322,共4页The Food Industry

基　　金：浙江省重点研发计划项目(2017C02033;2014C02012;2018C02053;2015C02053);浙江省自然科学基金资助项目(Q13C200006)

摘　　要：为探索简单、快速准确检测蛋黄粉中磷脂含量的方法,采用氯仿破坏脂质体,硫氰亚铁铵与磷脂反应生成配位化合物,在470 nm处检测其吸光度,计算其总磷脂含量。结果表明,在25℃条件下该方法在0.021~0.105 mg/mL范围内线性良好,平均加样回收率为99.84%(n=9),RSD为3.75%。硫氰亚铁铵比色法测定磷脂含量简单、快速,故此法适于蛋黄磷脂磷脂粗品总磷脂含量的快速检测分析。To explore a simple, rapid and accurate method for detecting content of phospholipids in egg yolk powder,liposomes were destroyed by chloroform, and ferricyanide was reacted with phospholipids to form coordination compounds. The absorbance was measured at 470 nm and the content of phospholipid was calculated. The results showed that the linearity was good in the range of 0.021-0.105 mg/mL. The average recoveries were 99.84%（n=9） and the RSD was 3.75%. The content was less in this method than the traditional molybdenum blue colorimetric method. The method of detecting phospholipids by thiocyanuric ammonium spectrophotometry was simple and rapid. Therefore, the method was suitable for rapid and crude analysis of total phospholipids content of crude egg yolk.

关 键 词：硫氰亚铁铵比色法 蛋黄卵磷脂 总磷脂 

分 类 号：O657.3[理学—分析化学] TS253.7[理学—化学]