DNA条形码技术鉴别市售鱼肉制品真伪  

The Authentication of Commercial Fish Products using DNA Barcoding Technique

作　　者：石蕊寒 南汇珠 丁红田 王建昌 付琦 孙晓霞 王金凤 丁丽丽[4] 崔景芳 刘洋 郭春海 SHI Ruihan;NAN Huizhu;DING Hongtian;WANG Jianchang;FU Qi;SUN Xiaoxia;WANG Jinfeng DING Lili;CUI Jingfang;LIU Yang;GUO Chunhai~＇;＊(Center of Inspection and Quarantine,Hebei Entry-Exit Inspection and Quarantine Bureau,Shijiazhuang 2.Hebei Academy of Inspection and Quarantine,Shijiazhuang 050051,China;3.Institute of Hebei Animal Health Supervision,Shijiazhuang 050081,Chin;4.Institute of Genetic Physiology,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,Chin;050051,China)

机构地区：[1]河此出入境检验检疫局技术中心,河北石家庄050051 [2]河北省检验检疫科学技术研究院,河北石家庄050051 [3]河北省动物卫生监督所,河北石家庄050081 [4]河北省农林科学院遗传生理研究所,河北石家庄050051

出　　处：《肉类研究》2018年第2期40-45,共6页Meat Research

基　　金：河北省科技计划项目(17275507D)

摘　　要：以COΙ基因和16S r RNA基因作为鱼类制品通用的DNA条形码,对超市中采集的91份冷冻鱼肉样品及经过深加工的预包装鱼肉样品进行物种鉴定。结果表明:COΙ基因和16S r RNA基因均可用于鱼类制品物种真伪的鉴别;冷冻鱼肉样品和预包装鱼肉样品与其商标的符合率分别为83.54%和58.33%;市场中存在鱼类制品商品标签标示错误、配料标注不明确等问题,为相关部门对鱼类制品的监管提供了有力的技术支持。This study was conducted to identify the species origin of 91 samples of frozen fish products and highly processed, pre-packaged fish products collected from supermarkets using DNA barcoding technique based on homologous sequence analysis of the CO1 and 16S rRNA genes. The results confirmed that both CO1 and 16S rRNA gene sequencing were useful for species authentication of fish products. The species origin of 66 of 79 （83.54%） frozen fish products and 7 of 12 （58.33%） highly processed, pre-packaged fish products was consistent with the product claims, which indicated that some problems such as mislabeling and ambiguous ingredient designation of fish products existed in the fish market. This research can provide technical support for the supervisory authorities concerned.

关 键 词：DNA条形码 C0I基因 16S RRNA基因 鱼肉制品 真伪鉴别 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]