基于RNA-Seq高通量测序技术的大鲵转录组分析  

Transcriptome Analysis of Chinese Giant Salamander(Andrias davidianus) Based on RNA-seq High-throughput Sequencing Technology

作　　者：黄勇[1] 高小蝉 孙西红[1] Huang Yong;Gao Xiaochan;Sun Xihong(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,471003)

机构地区：[1]河南科技大学动物科技学院

出　　处：《基因组学与应用生物学》2018年第9期3759-3768,共10页Genomics and Applied Biology

基　　金：河南省自然科学基金(No.162300410069);河南省高等学校重点科研项目(No.16A240002)共同资助

摘　　要：为发掘大鲵的功能基因,该研究以大鲵组织作为研究对象,利用RNA-seq技术对大鲵进行转录本测序和数据分析,经拼接组装共获得132 912条Unigenes,序列平均长度690 bp,N50为1 263 bp。另外从长度分布、GC含量与表达水平等方面对Unigenes进行评估,数据显示测序质量好,可信度高。此外,本研究也预测出132 416个能编码蛋白的Unigenes。使用9大数据库CDD、KOG、NR、NT、PFAM、Swiss-Prot、Tr EMBL、GO和KEGG注释大鲵转录组Unigenes,分别对应有24 049、18 406、36 711、15 858、20 500、27 515、36 705、28 879和10 958条Unigenes获得注释。其中,6 323条Unigenes在以上所有数据库中同时注释成功,39 672条Unigenes至少被一个数据库注释。KEGG分析结果显示：获得注释的10 958条Unigenes被划分到343个代谢通路中,参与信号转导通路的Unigenes数最多,共有2 644条（24.12%）;其次是免疫系统通路的Unigenes有2 450条（15.29%）。最后本研究还检测到了29 790个SSR位点。通过对大鲵转录组测序,获得了大量的转录组信息,有助于进一步研究大鲵功能基因克隆、基因组学、遗传多样性分析和分子标记开发等。In order to explore the function gene of Andrias davidianus, the tissue of A. davidianus was used as the research object, the transcript sequencing and data analysis were carried out technology. A total of 132 912 Unigenes were generated by de novo assembly with an average length of 690 bp and N50 of 1 263 bp. Unigenes qualities were assessed from the aspects of length distribution, GC content and expression level. The sequencing data was of high quality and reliability. In addition, 132 416 Unigenes that could encode protein were predicted.Using 9 databases（CDD, KOG, NR, NT, PFAM, Swissprot, Tr EMBL, GO and KEGG databases）, a total of 24 049,18 406, 36 711, 15 858, 20 500, 27 515, 36 705, 28 879 and 10 958 unigenes were annotated, respectively. Among them, 6 323 Unigenes were annotated successfully in all the above database at the same time, and 39 672 Unigenes were annotated in at least one database. The results of KEGG analysis showed that 10 958 annotated Unigenes were divided into 343 metabolic pathways. The number of Unigenes involved in the signal transduction pathway was maximum, with a total of 2 644（24.12%）, followed by 2 450（15.29%） Unigenes in the immune system pathway.Finally, 29 790 SSRs were also identified. A large amount of transcriptome information was obtained by RNA-seq of A. davidianus, which was beneficial to further research on functional gene cloning, genomics, genetic diversity analysis and molecular marker exploitation in A. davidianus.

关 键 词：大鲵 转录组 Unigenes 基因注释 

分 类 号：S917.4[农业科学—水产科学]