海地瓜多肽体外降糖活性评价  

Evaluation of hypoglycemic activity in vitro for polypeptides from Acaudina molpadioides

作　　者：王倩 扆雪涛 王宁丽[3,4] 裴栋 魏鉴腾[3,4] 邸多隆 王利涛[1] WANG Qian;YI Xue-tao;WANG Ning-li;PEI Dong;WEI Jian-teng;DI Duo-long;WANG Li-tao(College of Pharmacy,Jining Medical University,Jining 272013;Qingdao Institute for Food and Drug Control,Qingdao 266000;Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000;Qingdao Center of Resource Chemistry ＆ New Materials,Qingdao 266000)

机构地区：[1]济宁医学院药学院,济宁272013 [2]青岛市食品药品检验研究院,青岛266000 [3]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,兰州730000 [4]青岛市资源化学与新材料研究中心,青岛266000

出　　处：《食品科技》2018年第8期245-248,共4页Food Science and Technology

基　　金：国家重点研发计划项目(2017YFF0207800);中科院sTS项目(KFJ-SW-STS-146);宁波市科技富民项目(2016C10030).

摘　　要：目的:运用多种体外活性筛选方法评价海地瓜多肽的降糖活性,为海地瓜多肽进一步开发利用提供支持。方法:通过α-淀粉酶抑制剂筛选方法、DPP4抑制剂筛选方法及测定高糖损伤后胰岛细胞分泌胰岛素含量等多种体外降糖评价方法测定了海地瓜多肽的降糖活性。结果:多种体外降糖活性评价结果显示,海地瓜多肽可有效抑制α-淀粉酶活性、DPP4活性,同时可显著提升高糖损伤胰岛β细胞的胰岛素分泌量。结论:海地瓜多肽具有一定的降糖活性,可为后期海地瓜多肽的研发提供一定的理论支持。Objective： To evaluate the hypoglycemic activity for polypeptides from Acaudina molpadioides by a variety of active screening methods in vitro, and to provide support for the further development and utilization of polypeptides from Acaudina molpadioides. Methods： The hypoglycemic activity of polypeptides from Acaudina molpadioides were determined by a variety of hypoglycemic evaluation methods in vitro, such as screening of a-amylase inhibitor, screening of DPP4 inhibitor and determination of insulin secretion in islet cells after high glucose injury. Results： The evaluation results showed that the polypeptides from Acaudina molpadioides can inhibit the activity of a-amylase and DPP4 effectively, and can enhance the high glucose-induced pancreatic β-cell insulin secretion significantly. Conclusion： The polypeptides from Acaudina molpadioides has certain hypoglycemic activity, which may provide theoretical support for the further development of the polypeptides from Acaudina molpadioides.

关 键 词：海地瓜多肽 Α-淀粉酶抑制剂 DPP4抑制剂 胰岛素分泌量 

分 类 号：R915[医药卫生—微生物与生化药学]