N-myc downstream-regulated gene 2 promotes proliferation of HO-8910 ovarian cancer cells  

作　　者：Fenhong Kang Yaping Luo Yanlong Wang 

机构地区：[1]Department of Gynecology,Xiamen Maternal and Child Health Hospital

出　　处：《Oncology and Translational Medicine》2018年第4期171-175,共5页肿瘤学与转化医学（英文版）

摘　　要：Objective To investigate N-myc downstream-regulated gene 2(NDRG2) expression in ovarian cancer cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention.Methods Human NDRG2 L/S gene was obtained by revers-transcription polymerase chain reaction(RT-PCR). Sequence analysis confirmed the identity of NDRG2 L/S gene, which was then inserted into a eukaryotic vector p LNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells. Flow cytometry(FCM) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells transfected with p LNCX2-NDRG2 L/S was evaluated by FCM. Tumors were generated in female nude mice by subcutaneous injection of HO-8910 cells.Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2 expression positively correlated with the proliferation of HO-8910 cells. NDRG2 L/S promoted tumorigenicity in HO-8910 cells.Conclusion The present study identified a novel function of NDRG2 L/S gene and demonstrated its involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation and ovarian cancer progression.Objective To investigate N-myc downstream-regulated gene 2 （NDRG2） expression in ovarian cancer cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention. Methods Human NDRG2L/S gene was obtained by revers-transcription polymerase chain reaction （RT-PCR）. Sequence analysis confirmed the identity of NDRG2L/S gene, which was then inserted into a eukaryotic vector pLNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells. Flow cytometry （FCM） and 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） assay were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells transfected with pLNCX2-NDRG2L/S was evaluated by FCM. Tumors were generated in female nude mice by subcutaneous injection of HO-8910 cells. Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2 expression positively correlated with the proliferation of HO-8910 cells. NDRG2L/S promoted tumorigenicity in HO-8910 cells. Conclusion The present study identified a novel function of NDRG2L/S gene and demonstrated its involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation and ovarian cancer progression.

关 键 词：N-myc downstream-regulated gene 2 （NDRG2） ovarian cancer HO-8910 cell MTT CISPLATIN 

分 类 号：R737.31[医药卫生—肿瘤]