超声辅助紫萼玉簪根多糖提取工艺及抑菌研究  

Ultrasonic-assisted extraction process of polysaccharides from the root of Hosta ventricosa and its antibacterial research

作　　者：黄榕 王衍莉 包桥桥 刘回民[1] 刘树英[1] 刘洪章[1] HUANG Rong;WANG Yanli;BAO Qiaoqiao;LIU Huimin;LIU Shuying;LIU Hongzhang(Institute of Life Science,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院

出　　处：《黑龙江畜牧兽医》2018年第16期168-172,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家科技部成果转化项目(2014GB2B100007)

摘　　要：为了筛选紫萼玉簪根多糖的最佳提取条件,并研究紫萼玉簪根多糖抑菌活性,试验采用超声辅助法提取多糖,用硫酸苯酚法测定多糖含量,在料液比、超声次数、超声功率单因素试验的基础上,采用Box-Behnken响应面设计法建立数学模型,以确定最佳提取工艺;并测定紫萼玉簪根多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的最小抑菌浓度（MIC）。结果表明：紫萼玉簪根多糖在超声辅助提取中最佳提取工艺为超声功率390 W（52%）,料液比1∶93,超声次数128次（6 min 40 s）,多糖得率为23.6%,与理论值相差0.2百分点;紫萼玉簪根多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的MIC分别为18.75 mg/mL、37.5 mg/mL、75 mg/mL。In order to select the optimum extraction condition of polysaccharides from the root of Hosta ventricosa and to research its antibacterial activity, ultrasonic-assisted method was used in this experiment for the extraction of palysaccharides,and sulphuric acid-phenol method was adopted for the determination of polysaccharides content. Based on the data of single-factor tests including solid-liquid ratio, ultrasonic frequency and ultrasonic power, Box-Behnken response surface design method was used to establish the mathematical model and to determine optimum ex- traction process. The MIC values of polysaccharides from the root of Hosta ventricosa against Escherichia eoli ,Staphylococcus aureus and Bacillus subtilis were also measured. The results showed that ultrasonic-assisted optimum extraction condition of polysaccharides from the root of Hosta ventricosa was 390 W（ 52% ） for ultrasonic power, 1：93 for solid-liquid ratio, 128 （ 6 min 40 s） for ultrasonic frequency. Polysaccharide yield was 23.6% ,which had 0. 2 difference lower than the theoretical value. The MIC values of polysaccharides from the root of Hosta ventricosa against Escherichia coli,Staphylococcus aureus and Bacillus subtilis were 18.75 mg/mL,37.5 mg,/mL and 75 mg/mL respectively.

关 键 词：紫萼玉簪 超声辅助提取 多糖 响应面法 抑菌活性 

分 类 号：R284.2[医药卫生—中药学] O657.5[医药卫生—中医学]