大肠杆菌环境下EGCG的杀菌特性及其官能团变化  

BACTERICIDAL CHARACTERISTICS AND FUNCTIONAL GROUPS OF EGCG TO KILL ESCHERICHIA COLI

作　　者：郭子玉 冯萃敏[1,2] 汪长征[1,2] 韩芳[1,2] 谢寒 陈雪如 GUO Zi-yu;FENG Cui-min;WANG Chang-zheng;HAN Fang;XIE Han;CHEN Xue-ru(Beijing Advanced Innovation Center for Future Urban Design,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;National Demonstration Center for Experimental Water Environment Education（Beijing University of Civil Engineering and Architecture）,Beijing 100044,China;Xiangyang Vocational and Technical College,441050,China;Beijing General Municipal Engineering Design ＆ Research Institute Co.,Ltd,Bcijing 100082,China)

机构地区：[1]北京建筑大学北京未来城市设计高精尖创新中心,北京100044 [2]水环境国家级实验教学示范中心(北京建筑大学),北京100044 [3]襄阳职业技术学院,441050 [4]北京市市政工程设计研究总院有限公司,北京100082

出　　处：《环境工程》2018年第8期43-47,共5页Environmental Engineering

基　　金：国家自然科学基金项目(51678026);北京建筑大学市属高校基本科研业务费专项资金(X18279);北京市科技计划课题(Z171100000717011)

摘　　要：通过测定EGCG对大肠杆菌杀灭过程中的Zeta电位和颗粒粒径,判断了EGCG和大肠杆菌的结合特性;通过傅里叶红外光谱法测定了EGCG和大肠杆菌作用前后的官能团变化,确定大肠杆菌细胞的破坏情况及EGCG起主要杀菌作用的官能团。结果表明:EGCG和大肠杆菌的电荷属性有利于EGCG发挥杀菌性能;离子的存在会影响EGCG的活性官能团;EGCG杀灭大肠杆菌的机理是先通过其结构中酚羟基的氢键与碱性氨基酸侧链的氮原子相互作用,嵌入大肠杆菌细胞内,抑制大肠杆菌细胞膜孔蛋白的功能,最终导致大肠杆菌细胞死亡。Disinfection is the key measure to ensure the safety of drinking water. The common disinfection methods have some limitations, such as disinfection by-products. At present, researchers applied the green resource polyphenols, which had various physiological functions and wide antimicrobial activities, to the disinfection of drinking water, and confirmed the bactericidal and bacteriostatic properties of tea polyphenols and the practicability of disinfection of drinking water. By measuring EGCG of Zeta potential and particle killing Escherichia coli in the process of particle size, the killing characteristics of EGCG and Escherichia eoli were studied, its functional groups variation before and after EGCG killing Eseherichia coli by Fourier transform infrared spectroscopy was determined for evsuring the functional destruction of Escherichia coli cells, and the main groups of EGCG played a bactericidal effect. The results showed that the charge properties of EGCG and Eseherichia coli was conducive to play a bactericidal performance; ion could influence the activity of functional groups of EGCG; the mechanism of EGCG killing Escherichia eoli was that hydrogen bonded through the nitrogen atom and basic amino acid side chain of phenolic hydroxyl groups in the structure of the interaction, and embedded in Eseherichia coli cells. The main function inhibition of Escherichia coli cell membrane protein resulted in cell death of Escherichia coll.

关 键 词：茶多酚 EGCG 大肠杆菌 杀菌 官能团 

分 类 号：TU991.2[建筑科学—市政工程]