鸡血藤提取物的TLC鉴别及儿茶素和表儿茶素的含量测定  

TLC Identification and Determination of Catechin and Epicatechin in Spatholobi Caulis Extract

作　　者：陆雪丽 潘晓鹃[1,2] 邓萌萌 吴佳欣[1,2] 赵俊红 LU Xue-li;PAN Xiao-juan;DENG Meng-meng;WU Jia-xin;ZHAO Jun-hong(School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]四川省中医药科学院,成都610041

出　　处：《中国实验方剂学杂志》2018年第18期88-92,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省科研院所科技成果转化项目(14010101);四川省公益性科研院所基本科研业务费项目(A-2013N-22)

摘　　要：目的：建立鸡血藤提取物的薄层色谱法（TLC）鉴别及其有效成分儿茶素和表儿茶素总量的高效液相色谱法（HPLC）测定,为该药材的质量控制提供参考。方法：TLC以0.7%羧甲基纤维素钠硅胶G作为吸附剂,三氯甲烷-丙酮-正丁醇-甲醇-甲酸（67∶13∶14∶3∶3）为展开剂,5%香草醛硫酸溶液为显色剂。儿茶素、表儿茶素总量测定的HPLC采用KinetexC18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.02%磷酸水溶液为流动相进行梯度洗脱,流速1.0 m L·min-1,柱温25℃,进样量5μL,检测波长280 nm。结果：鸡血藤提取物的TLC中,在与儿茶素、表儿茶素对照品相对应的位置上显相同颜色、相同形状的斑点,且斑点清晰、分离度好。在HPLC定量分析中,儿茶素、表儿茶素的线性范围（质量浓度与峰面积积分值）分别为9.9-198.8,20.6-412.4 mg·L-1,相关系数均为0.999 9;儿茶素、表儿茶素的平均回收率分别为98.12%和97.94%,RSD分别为1.7%和1.4%。结论：该研究建立的TLC专属性强、分离度好;建立的测定儿茶素、表儿茶素总量的HPLC准确度高、重复性好,可用于鸡血藤提取物的质量控制。Objective： To identify Spatholobi Caulis extract with thin layer chromatography（ TLC） and determine total content of catechin and epicatechin in it with high performance liquid chromatography（ HPLC）,in order to provide a reference for the quality control of Spatholobi Caulis. Method： TLC was put into effect with0. 7% sodium carboxymethylcellulose（ CMC-Na） silica gel G as adsorbent,chloroform-acetone-（ n-butanol）-methanol-formic acid（ 67 ∶ 13 ∶ 14 ∶ 3 ∶ 3） as developing solvent and 5% vanillin sulfuric acid solution as chromogenic agent. HPLC was carried out with the Kinetex  C18 column（ 4. 6 mm × 250 mm,5 μm） and methanol-0. 02% phosphoric acid water solution as mobile phase to perform gradient elution; the flow rate was1. 0 m L·min-1,the column temperature was 25 ℃,the injection volume was 5 μL,and the detection wavelength was 280 nm. Result： In the TLC,the color and shape of the extract spots were the same as those of catechin and epicatechin references at the corresponding positions, and those spots were clear and well separated. In the quantitative analysis of HPLC, catechin and epicatechin had good linear relationships in the range of 9. 9-198. 8 mg·L-1 and 20. 6-412. 4 mg·L-1,respectively; and their correlation coefficients were all 0. 999 9. The average recoveries of catechin and epicatechin was 98. 12% and 97. 94% with RSD of 1. 7% and 1. 4%,respectively. Conclusion： The TLC established in this experiment has strong specificity and good separating degree. The HPLC which was used to determine total content of catechin and epicatechin in the extract has high accuracy and good repeatability. The established methods can be used to control quality of Spatholobi Caulis extract.

关 键 词：鸡血藤 提取物 薄层色谱法 儿茶素 表儿茶素 含量测定 

分 类 号：R22[医药卫生—中医基础理论] R282[医药卫生—中医学]