Novel sesquiterpenoids isolated from Chimonanthus praecox and their antibacterial activities  

作　　者：LOU Hua-Yong ZHANG Yu MA Xiao-Pan JIANG Sai WANG Xiang-Pei YI Ping LIANG Guang-Yi WU Hong-Mei FENG Jing JIN Feng-Yun PAN Wei-Dong 

机构地区：[1]Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China [2]State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China [3]The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China

出　　处：《Chinese Journal of Natural Medicines》2018年第8期621-627,共7页中国天然药物（英文版）

基　　金：supported by the Ministry of Science&Technology on China(No.2017YFD0201402);the National Natural Science Foundation of China(Nos.81160390 and 81660580);the Science and Technology Department of Guizhou Province,China{Nos.(QKHRCTD[2015]4026);(QKHLHZ[2015]7400);QKHRC[2016]4037,[2011]LKZ7051,and QKZHJ(2009)2150}

摘　　要：In the present study,four new sesquiterpenoids,chimonols A–D(compounds 1–4),together with four known compounds(5–8) were isolated from the Et OAc extract of Chimonanthus praecox Link.The structures of these new compounds were elucidated on the basis of spectroscopic techniques(UV,IR,MS,and 1 D and 2 D NMR),and their absolute configurations were established by comparing experimental and calculated electronic circular dichroism(ECD) spectra.Compounds 1–8 were evaluated for antimicrobial activities and the minimum inhibitory concentrations(MICs) were determined by the broth microdilution method in 96-well culture plates.Compounds 1,2,and 7 exhibited weak antibacterial effects for S.aureus(ATCC 6538),E.coli(ATCC 11775),and P.aeruginosa(ATCC 10145) with MIC values being 158–249 μg·mL^(-1).Compounds 3–7 showed activities against C.glabrata(ATCC 2001) and S.aureus(ATCC 43300) with MIC values being 128–197 μg·mL^(-1).Compounds 1–4 showed activity against S.aureus(ATCC 25923) with MIC values being 162–254 μg·mL^(-1).The present study provided a basis for future evaluation of these compounds as antibacterial agents.In the present study,four new sesquiterpenoids,chimonols A–D（compounds 1–4）,together with four known compounds（5–8） were isolated from the Et OAc extract of Chimonanthus praecox Link.The structures of these new compounds were elucidated on the basis of spectroscopic techniques（UV,IR,MS,and 1 D and 2 D NMR）,and their absolute configurations were established by comparing experimental and calculated electronic circular dichroism（ECD） spectra.Compounds 1–8 were evaluated for antimicrobial activities and the minimum inhibitory concentrations（MICs） were determined by the broth microdilution method in 96-well culture plates.Compounds 1,2,and 7 exhibited weak antibacterial effects for S.aureus（ATCC 6538）,E.coli（ATCC 11775）,and P.aeruginosa（ATCC 10145） with MIC values being 158–249 μg·mL^（-1）.Compounds 3–7 showed activities against C.glabrata（ATCC 2001） and S.aureus（ATCC 43300） with MIC values being 128–197 μg·mL^（-1）.Compounds 1–4 showed activity against S.aureus（ATCC 25923） with MIC values being 162–254 μg·mL^（-1）.The present study provided a basis for future evaluation of these compounds as antibacterial agents.

关 键 词：SESQUITERPENOIDS CHIMONANTHUS praecox Electronic CIRCULAR DICHROISM ANTIMICROBIAL activities 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]