液相色谱-串联质谱测定葡萄酒中4-乙基苯酚和4-乙基愈创木酚  

Determination of 4-ethylphenol and 4-ethylguaiacol in wine by liquid chromatography-tandem mass spectrometry

作　　者：陈树兵[1] 刘忠义 陈颖 方科益[1] 王传现[2] 张敏超 李晓虹[2] CHEN Shu-Bing;LIU Zhong-Yi;CHEN Yin;FANG Ke-Yi;WANG Chuan-Xian;ZHANG Min-Chao;LI Xiao-Hong(Ningbo Entry-Exit Inspection and Quarantine Bureau Technology Center,Ningbo 315012,China;Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200315,China)

机构地区：[1]宁波出入境检验检疫局检验检疫技术中心,宁波315012 [2]上海出入境检验检疫局,上海200315

出　　处：《食品安全质量检测学报》2018年第16期4407-4411,共5页Journal of Food Safety and Quality

基　　金：国家质检总局科技项目(2016IK169);国家质检总局科技计划项目(2015IK226);保税区管理局自贸区专项发展资金项目(中(沪)自保管[2016]192号);上海市科委技术标准专项项目(14DZ0501001)~~

摘　　要：目的建立液相色谱-串联质谱法同时测定葡萄酒中4-乙基苯酚和4-乙基愈创木酚的方法。方法样品经甲醇(1.0%甲酸)溶液提取,Oasis HLB固相萃取柱净化后,采用液相色谱-串联质谱法负离子模式检测,通过多反应监测模式,对葡萄酒中4-乙基苯酚和4-乙基愈创木酚进行定量分析。结果 4-乙基苯酚和4-乙基愈创木酚线性范围为0.01～0.1μg/m L,定量限(limit of quantification,LOQ)为0.01μg/kg,加标回收率为77.6%～93.5%,相对标准偏差均小于10%。结论本方法操作简单,结果准确可靠,对葡萄酒中4-乙基苯酚和4-乙基愈创木酚的检测具有较强的适用性。Objective To establish a method for determination of 4-ethylphenol and 4-ethylguaiacol in wine by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. Methods The samples were extracted with methyl alcohol（1% formic acid）, and then purified by the Oasis HLB SPE column. Finally the 4-ethylphenol and 4-ethylguaiacol in wine were measured by LC-MS/MS using multiple reaction monitoring（MRM） with negative ion mode. Results The 4-ethylphenol and 4-ethylguaiacol showed a good linear relationship in the concentration range of 0.01-0.1 μg/m L, the limit of quantification（LOQ） was 0.01 μg/kg, the average recoveries were 77.6%-93.5%, and the relative standard deviation（RSD） was not more than 10%. Conclusion This method is simple and accurate, which is suitable for determination of 4-ethylphenol and 4-ethylguaiacol in wine.which is suitable for determination of 4-ethylphenol and 4-ethylguaiacol in wine.

关 键 词：葡萄酒 4-乙基苯酚 4-乙基愈创木酚 液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TS262.6[理学—化学]